Source:http://linkedlifedata.com/resource/pubmed/id/18089306
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
10
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pubmed:dateCreated |
2007-12-19
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pubmed:abstractText |
Nitric oxide (NO), produced by nitric oxide synthase, is implicated in the pathophysiology of renal ischemia/reperfusion (I/R) injury. This study sought to elucidate the impact of pharmacological induction of heme oxygenase-1 (HO-1) on renal I/R injury. Rats were subjected to 45 minutes of renal ischemia followed by various times of reperfusion (30 minutes, 1 hour, or 3 hours). Plasma from sacrificed rats was obtained, and the kidneys processed for the expression of iNOS, cleaved caspase-3, p38MAPK and for immunohistochemical analysis. Furthermore, we determined renal and plasma levels of lipid hydroperoxides, total thiol groups, and plasmatic NO2-/NO3- formation. Our results showed a time-dependent increase in iNOS expression, which was also confirmed by increased plasma formation of NO2-/NO3-. Interestingly, this effect was reversed by pretreatment (12 hours) with SnCl2, a potent and specific inducer of renal HO-1 expression and activity, or by intraperitoneal injection of biliverdin (10 mg/kg). Furthermore, we observed a concomitant reduction in plasma and renal LOOH formation, a normalization of renal total thiol content, a reduction of caspase-3-mediated apoptosis, and a significant increase in p38MAPK phosphoration. Taken together, these results suggested that HO-1 and its byproduct biliverdin play major roles in the pathophysiological cascade leading to renal I/R injury.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Heme Oxygenase-1,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrates,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type II,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrites,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfhydryl Compounds
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0041-1345
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
39
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2986-91
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pubmed:meshHeading |
pubmed-meshheading:18089306-Animals,
pubmed-meshheading:18089306-Cyclooxygenase Inhibitors,
pubmed-meshheading:18089306-Disease Models, Animal,
pubmed-meshheading:18089306-Enzyme Induction,
pubmed-meshheading:18089306-Heme Oxygenase-1,
pubmed-meshheading:18089306-Isoenzymes,
pubmed-meshheading:18089306-Nitrates,
pubmed-meshheading:18089306-Nitric Oxide Synthase Type II,
pubmed-meshheading:18089306-Nitrites,
pubmed-meshheading:18089306-Oxidative Stress,
pubmed-meshheading:18089306-Rats,
pubmed-meshheading:18089306-Renal Circulation,
pubmed-meshheading:18089306-Reperfusion Injury,
pubmed-meshheading:18089306-Sulfhydryl Compounds
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pubmed:year |
2007
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pubmed:articleTitle |
Pharmacological induction of heme oxygenase-1 inhibits iNOS and oxidative stress in renal ischemia-reperfusion injury.
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pubmed:affiliation |
Department of Biological Chemistry, University of Catania, Catania, Italy. livolti@unict.it
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pubmed:publicationType |
Journal Article
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