Linked Life Data

18088183

Source:http://linkedlifedata.com/resource/pubmed/id/18088183

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2007-12-19
pubmed:abstractText
We studied the induction of apoptosis in Jurkat cells by UVB radiation (wavelength 290-320 nm) at a dose of 310 mJ/ cm2. We combined Fourier transform infrared (FTIR) spectroscopy with flow cytometry to determine whether the combination of both techniques could provide new and improved information about cell modifications. To do this, we looked for correspondences and correlations between spectroscopy and flow cytometry data and found three highly probable spectroscopic markers of apoptosis. The behavior of the wave number shift of both the Amide I beta-sheet component and the area of the 1083 cm(-1) band reproduced, with a high correlation, the behavior of the early apoptotic cell population, while the behavior of the Amide I area showed a high correlation with the early plus late apoptotic cell population.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0033-7587
pubmed:author
pubmed:issnType
Print
pubmed:volume
168
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
698-705
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
UVB-radiation-induced apoptosis in Jurkat cells: a coordinated fourier transform infrared spectroscopy-flow cytometry study.
pubmed:affiliation
Dipartimento di Medicina Sperimentale e Patologia, Università di Roma La Sapienza, Roma, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't