|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
2008-1-30
|
|
pubmed:abstractText |
Bacteria contain a single multisubunit RNA polymerase that is responsible for the synthesis of all RNA. Previous studies of the Escherichia coli K-12 laboratory strain identified a group of effector proteins that interact directly with RNA polymerase to modulate the efficiency of transcription initiation, elongation, or termination. Here we used a rapid affinity isolation technique to isolate RNA polymerase from the pathogenic Escherichia coli strain O157:H7 Sakai. We analyzed the RNA polymerase enzyme complex using mass spectrometry and identified associated proteins. Although E. coli O157:H7 Sakai contains more than 1,600 genes not present in the K-12 strain, many of which are predicted to be involved in transcription regulation, all of the identified proteins in this study were encoded on the "core" E. coli genome.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18083804-10320479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18083804-10734204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18083804-10833023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18083804-10857624,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18083804-10982868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18083804-11258796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18083804-11605791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18083804-11721901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18083804-12065489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18083804-12471157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18083804-12853150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18083804-14630944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18083804-15690043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18083804-16155292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18083804-16212429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18083804-16606699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18083804-16980472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18083804-17332743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18083804-17506902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18083804-1831176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18083804-3936407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18083804-4882047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18083804-6283015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18083804-7608084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18083804-8557736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18083804-9507009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18083804-9614128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18083804-9777854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18083804-9893021
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
1098-5530
|
|
pubmed:author |
|
|
pubmed:issnType |
Electronic
|
|
pubmed:volume |
190
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1284-9
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:18083804-Blotting, Western,
pubmed-meshheading:18083804-Chromatography, Affinity,
pubmed-meshheading:18083804-DNA-Directed RNA Polymerases,
pubmed-meshheading:18083804-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:18083804-Escherichia coli O157,
pubmed-meshheading:18083804-Escherichia coli Proteins,
pubmed-meshheading:18083804-Gene Expression Regulation, Bacterial,
pubmed-meshheading:18083804-Mass Spectrometry,
pubmed-meshheading:18083804-Models, Biological,
pubmed-meshheading:18083804-Protein Binding,
pubmed-meshheading:18083804-Protein Subunits,
pubmed-meshheading:18083804-Spectrometry, Mass, Matrix-Assisted Laser...,
pubmed-meshheading:18083804-Tandem Mass Spectrometry
|
|
pubmed:year |
2008
|
|
pubmed:articleTitle |
Affinity isolation and I-DIRT mass spectrometric analysis of the Escherichia coli O157:H7 Sakai RNA polymerase complex.
|
|
pubmed:affiliation |
School of Biosciences, University of Birmingham, Birmingham B15 2TT, United Kingdom. D.lee@bham.ac.uk
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|
