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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2008-1-21
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pubmed:abstractText |
Demineralized bone matrix (DBM) is a collagen-based scaffold, but its low mechanical strength and limited BMP-2 binding ability restrict its application in bone repair. It is known that heparin could be immobilized onto scaffolds to enhance their binding of growth factors with the heparin-binding domain. Here, we crosslinked heparin to DBM to increase its BMP-2 binding ability. To our surprise, the mechanical strength of DBM was also dramatically increased. The compression modulus of heparin crosslinked DBM (HC-DBM) have improved (seven-fold increased) under wet condition, which would allow the scaffolds to keep specific shapes in vivo. As expected, HC-DBM showed specific binding ability to BMP-2. Additional studies showed the bound BMP-2 exerted its function to induce cell differentiation on the scaffold. Subcutaneous implantation of HC-DBM carrying BMP-2 showed higher alkaline phosphatase (ALP) activity (2 weeks), more calcium deposition (4 and 8 weeks) and more bone formation than that of control groups. It is concluded that HC-DBM has increased mechanical intensity as well as specific BMP-2 binding ability; HC-DBM/BMP-2 enhances the osteogenesis and therefore could be an effective medical device for bone repair.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Alkaline Phosphatase,
http://linkedlifedata.com/resource/pubmed/chemical/BMP2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Biocompatible Materials,
http://linkedlifedata.com/resource/pubmed/chemical/Bmp2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Protein 2,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Substitutes,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Cross-Linking Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/Heparin,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0142-9612
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
29
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1189-97
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:18083224-Alkaline Phosphatase,
pubmed-meshheading:18083224-Animals,
pubmed-meshheading:18083224-Biocompatible Materials,
pubmed-meshheading:18083224-Biomechanics,
pubmed-meshheading:18083224-Bone Matrix,
pubmed-meshheading:18083224-Bone Morphogenetic Protein 2,
pubmed-meshheading:18083224-Bone Morphogenetic Proteins,
pubmed-meshheading:18083224-Bone Substitutes,
pubmed-meshheading:18083224-Calcium,
pubmed-meshheading:18083224-Cattle,
pubmed-meshheading:18083224-Cross-Linking Reagents,
pubmed-meshheading:18083224-Heparin,
pubmed-meshheading:18083224-Humans,
pubmed-meshheading:18083224-Male,
pubmed-meshheading:18083224-Materials Testing,
pubmed-meshheading:18083224-Microscopy, Electron, Scanning,
pubmed-meshheading:18083224-Osteogenesis,
pubmed-meshheading:18083224-Protein Binding,
pubmed-meshheading:18083224-Rats,
pubmed-meshheading:18083224-Rats, Sprague-Dawley,
pubmed-meshheading:18083224-Tissue Engineering,
pubmed-meshheading:18083224-Transforming Growth Factor beta
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pubmed:year |
2008
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pubmed:articleTitle |
The effect of crosslinking heparin to demineralized bone matrix on mechanical strength and specific binding to human bone morphogenetic protein-2.
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pubmed:affiliation |
Key Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 3 Nanyitiao, Zhongguancun, Beijing, China.
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pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro,
Research Support, Non-U.S. Gov't
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