Linked Life Data

18082938

Source:http://linkedlifedata.com/resource/pubmed/id/18082938

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2008-2-11
pubmed:abstractText
This work proposed a method to assess the occult micrometastasis characteristic in peri-operative peripheral blood (PB) based on multipoint quantification of multimarker genes by real-time semiquantitative RT-PCR. The expression levels of SCC, CK19, CK20, CEA and survivin mRNA in PB samples collected before surgery (B-1), immediately after surgery (B0) and at the third day post-operatively (B+3) from 36 squamous esophageal cancer (EC) patients were detected. SCC and CK19 mRNA showed low positivity detection rates, while the rate for CEA and survivin mRNA panel was 58.3%, 83.3% and 72.3% at B-1, B0 and B+3, respectively. Opposite to the significant increase and slow decrease of CEA cells at stages of B-1 to B0 and B0 to B+3, respectively, survivin cells decreased significantly and increased quickly at the two stages. The follow-up with a period of 1.2 years showed that the patients with the B+3/B0 ratios of >or= 0.3 for CEA cells or/and >or= 10 for survivin cells exhibited significantly high possibility of developed metastasis, and the sensitivity to predict developed metastasis increased from 54.5% of CEA mRNA alone to 72.7% of CEA and survivin mRNA panel. These results suggested that CEA and survivin gene panel improved the sensitivity to predict recurrence and differentiate the micrometastatic process.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0304-3835
pubmed:author
pubmed:issnType
Print
pubmed:day
8
pubmed:volume
261
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
46-54
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Multipoint quantification of multimarker genes in peripheral blood and micrometastasis characteristic in peri-operative esophageal cancer patients.
pubmed:affiliation
MOE Key Laboratory of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Nanjing 210093, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't