| pubmed-article:18082422 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18082422 | lifeskim:mentions | umls-concept:C0004651 | lld:lifeskim |
| pubmed-article:18082422 | lifeskim:mentions | umls-concept:C0751282 | lld:lifeskim |
| pubmed-article:18082422 | lifeskim:mentions | umls-concept:C0036584 | lld:lifeskim |
| pubmed-article:18082422 | lifeskim:mentions | umls-concept:C0678594 | lld:lifeskim |
| pubmed-article:18082422 | lifeskim:mentions | umls-concept:C1548779 | lld:lifeskim |
| pubmed-article:18082422 | lifeskim:mentions | umls-concept:C1947902 | lld:lifeskim |
| pubmed-article:18082422 | lifeskim:mentions | umls-concept:C2827499 | lld:lifeskim |
| pubmed-article:18082422 | lifeskim:mentions | umls-concept:C0205460 | lld:lifeskim |
| pubmed-article:18082422 | lifeskim:mentions | umls-concept:C1167624 | lld:lifeskim |
| pubmed-article:18082422 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:18082422 | pubmed:dateCreated | 2008-1-21 | lld:pubmed |
| pubmed-article:18082422 | pubmed:abstractText | There is a need for improved tools for labeling protein species within large macromolecular assemblies. Here we describe a method for the efficient selenomethionine labeling of the membrane-containing bacterial virus PM2 for structural studies. By examining potential host cells a strain was found which was auxotrophic for methionine, and by performing a multiparameter search of conditions it was possible to derive a robust protocol which simultaneously minimized the toxic effects of the selenomethionine, so that a reasonable virus yield was maintained, whilst still achieving essentially complete labeling. This has allowed us to fingerprint the protein constituents of the virus in a relatively low resolution electron density map. Such a technique can be adapted to other macromolecule complexes studied by X-ray crystallography. | lld:pubmed |
| pubmed-article:18082422 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18082422 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18082422 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18082422 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18082422 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18082422 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:18082422 | pubmed:issn | 1095-8657 | lld:pubmed |
| pubmed-article:18082422 | pubmed:author | pubmed-author:BamfordDennis... | lld:pubmed |
| pubmed-article:18082422 | pubmed:author | pubmed-author:BamfordJaana... | lld:pubmed |
| pubmed-article:18082422 | pubmed:author | pubmed-author:StuartDavid... | lld:pubmed |
| pubmed-article:18082422 | pubmed:author | pubmed-author:AbresciaNicol... | lld:pubmed |
| pubmed-article:18082422 | pubmed:author | pubmed-author:KiveläHanna... | lld:pubmed |
| pubmed-article:18082422 | pubmed:author | pubmed-author:GrimesJonatha... | lld:pubmed |
| pubmed-article:18082422 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:18082422 | pubmed:volume | 161 | lld:pubmed |
| pubmed-article:18082422 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18082422 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18082422 | pubmed:pagination | 204-10 | lld:pubmed |
| pubmed-article:18082422 | pubmed:meshHeading | pubmed-meshheading:18082422... | lld:pubmed |
| pubmed-article:18082422 | pubmed:meshHeading | pubmed-meshheading:18082422... | lld:pubmed |
| pubmed-article:18082422 | pubmed:meshHeading | pubmed-meshheading:18082422... | lld:pubmed |
| pubmed-article:18082422 | pubmed:meshHeading | pubmed-meshheading:18082422... | lld:pubmed |
| pubmed-article:18082422 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18082422 | pubmed:articleTitle | Selenomethionine labeling of large biological macromolecular complexes: probing the structure of marine bacterial virus PM2. | lld:pubmed |
| pubmed-article:18082422 | pubmed:affiliation | Department of Biological and Environmental Science and Nanoscience Centre, University of Jyväskylä, P.O. Box 35 (Survontie 9), 40014 Jyväskylä, Finland. | lld:pubmed |
| pubmed-article:18082422 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18082422 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:18082422 | lld:pubmed |
