Linked Life Data

18082422

Source:http://linkedlifedata.com/resource/pubmed/id/18082422

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2008-1-21
pubmed:abstractText
There is a need for improved tools for labeling protein species within large macromolecular assemblies. Here we describe a method for the efficient selenomethionine labeling of the membrane-containing bacterial virus PM2 for structural studies. By examining potential host cells a strain was found which was auxotrophic for methionine, and by performing a multiparameter search of conditions it was possible to derive a robust protocol which simultaneously minimized the toxic effects of the selenomethionine, so that a reasonable virus yield was maintained, whilst still achieving essentially complete labeling. This has allowed us to fingerprint the protein constituents of the virus in a relatively low resolution electron density map. Such a technique can be adapted to other macromolecule complexes studied by X-ray crystallography.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1095-8657
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
161
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
204-10
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Selenomethionine labeling of large biological macromolecular complexes: probing the structure of marine bacterial virus PM2.
pubmed:affiliation
Department of Biological and Environmental Science and Nanoscience Centre, University of Jyväskylä, P.O. Box 35 (Survontie 9), 40014 Jyväskylä, Finland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't