Linked Life Data

18081320

Source:http://linkedlifedata.com/resource/pubmed/id/18081320

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2008-1-3
pubmed:databankReference
pubmed:abstractText
Pseudomonas aeruginosa PA4872 was identified by sequence analysis as a structurally and functionally novel member of the PEP mutase/isocitrate lyase superfamily and therefore targeted for investigation. Substrate screens ruled out overlap with known catalytic functions of superfamily members. The crystal structure of PA4872 in complex with oxalate (a stable analogue of the shared family alpha-oxyanion carboxylate intermediate/transition state) and Mg2+ was determined at 1.9 A resolution. As with other PEP mutase/isocitrate lyase superfamily members, the protein assembles into a dimer of dimers with each subunit adopting an alpha/beta barrel fold and two subunits swapping their barrel's C-terminal alpha-helices. Mg2+ and oxalate bind in the same manner as observed with other superfamily members. The active site gating loop, known to play a catalytic role in the PEP mutase and lyase branches of the superfamily, adopts an open conformation. The Nepsilon of His235, an invariant residue in the PA4872 sequence family, is oriented toward a C(2) oxygen of oxalate analogous to the C(3) of a pyruvyl moiety. Deuterium exchange into alpha-oxocarboxylate-containing compounds was confirmed by 1H NMR spectroscopy. Having ruled out known activities, the involvement of a pyruvate enolate intermediate suggested a decarboxylase activity of an alpha-oxocarboxylate substrate. Enzymatic assays led to the discovery that PA4872 decarboxylates oxaloacetate (kcat = 7500 s(-1) and Km = 2.2 mM) and 3-methyloxaloacetate (kcat = 250 s(-1) and Km = 0.63 mM). Genome context of the fourteen sequence family members indicates that the enzyme is used by select group of Gram-negative bacteria to maintain cellular concentrations of bicarbonate and pyruvate; however the decarboxylation activity cannot be attributed to a pathway common to the various bacterial species.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-10089316, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-10378273, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-10531521, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-10801489, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-10932251, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-11526312, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-12162742, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-12351820, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-12393928, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-1244355, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-12499537, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-12515554, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-12706720, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-12842039, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-12906829, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-14575713, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-14596586, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-14617778, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-15078090, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-15251467, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-15299374, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-15299456, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-15299926, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-15572783, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-15723538, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-16342929, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-16342930, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-16381885, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-16981709, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-17244616, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-17270211, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-179820, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-2025413, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-3741834, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-566117, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-7012893, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-8172901, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-9468496, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081320-9757107
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
8
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
167-82
pubmed:dateRevised
2011-5-5
pubmed:meshHeading
More...