Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2007-12-26
pubmed:abstractText
The inhibitory effect of DAP12 on macrophages has been revealed by examining myeloid cells from DAP12-deficient mice. In this report, we demonstrate that both DAP12 and the FcepsilonRIgamma-chain (FcRgamma) are required for negative regulation of TLR responses in bone marrow-derived dendritic cells (DC). Loss of both DAP12 and FcRgamma enhanced the pro-inflammatory cytokine production and maturation of DC after TLR stimulation, resulting in a greater percentage of DC that produced IL-12 p40, TNF, and IL-6, and expressed high levels of MHC class II, CD80, and CD86. Whereas DC lacking only DAP12 showed some increased TLR responses, those lacking only FcRgamma had a greater enhancement of maturation and cytokine production, though to a lesser extent than DC lacking both DAP12 and FcRgamma. Additionally, antigen-specific T cell proliferation was enhanced by DAP12(-/-)FcRgamma(-/-) DC relative to wild-type DC after maturation. Similar to DAP12(-/-)FcRgamma(-/-) DC, Syk-deficient DC also had increased inflammatory cytokine production, maturation, and antigen presentation. These results confirm the inhibitory effect of immunoreceptor tyrosine-based activation motif (ITAM) signaling in myeloid cells and show that DC and macrophages differ in their dependence on the ITAM-containing adapters DAP12 and FcRgamma for negative regulation of TLR signaling.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-10604985, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-11021532, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-11021533, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-11602640, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-11970878, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-12777403, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-15073337, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-15650060, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-15664157, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-15884055, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-15895090, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-15941912, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-16116174, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-16206234, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-16227091, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-16293595, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-16313340, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-16341139, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-16449667, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-16691244, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-16735691, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-16783855, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-16887962, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-16920926, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-16951310, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-17100880, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-17110943, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-17202337, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-17220916, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-17223960, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-17227821, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-17275323, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-17328678, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-17356173, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-17370119, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-17476349, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-17479101, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-17486093, http://linkedlifedata.com/resource/pubmed/commentcorrection/18081038-8313472
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0014-2980
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
166-73
pubmed:dateRevised
2011-9-26
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Increased TLR responses in dendritic cells lacking the ITAM-containing adapters DAP12 and FcRgamma.
pubmed:affiliation
Immunology Research Center, National Health Research Institutes, Taiwan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't
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