Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2008-1-24
pubmed:abstractText
The global regulatory system of Escherichia coli, carbon storage regulator (Csr), was engineered to increase the intracellular concentration of phosphoenolpyruvate. We examined the effects of csrA and csrD mutations and csrB overexpression on phenylalanine production in E. coli NST37 (NST). Overexpression of csrB led to significantly greater phenylalanine production than csrA and csrD mutations (2.33 vs 1.67 and 1.61 g l(-1), respectively; P < 0.01). Furthermore, the overexpression of csrB was confirmed by the observed increase in csrB transcription level. We also determined the effect of overexpressing transketolase A (TktA) or glucose-6-phosphate dehydrogenase (Zwf) in NST and the csrA mutant of NST (NSTCSRA) on phenylalanine production. The NSTCSRA strain overexpressing TktA (NSTCSRA [pTktA]) produced significantly more phenylalanine than that of Zwf (2.39 vs 1.61 g l(-1); P > 0.01). Furthermore, we examined the effect of overexpressing TktA, 3-deoxy-D: -arabino-heptulosonate-7-phosphate synthase (AroF(FR)), and chorismate mutase/prephenate dehydratase (PheA(FR)) together in NSTCSRA (NSTCSRA [pTkaFpA]). It is interesting to note that NSTCSRA [pTkaFpA] produced significantly less phenylalanine than both NSTCSRA [pTktA] and NST overexpressing csrB (NST [pCsrB]) (1.84 vs 2.39 and 2.33 g l(-1), respectively; P < 0.01). Thus, csrB overexpression or csrA mutation in combination with tktA overexpression was more effective than previous approaches that targeted the glycolytic or aromatic pathway enzymes for enhancing phenylalanine production.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/3-Deoxy-7-Phosphoheptulonate..., http://linkedlifedata.com/resource/pubmed/chemical/Chorismate Mutase, http://linkedlifedata.com/resource/pubmed/chemical/CsrA protein, E coli, http://linkedlifedata.com/resource/pubmed/chemical/CsrB RNA, E coli, http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Glucosephosphate Dehydrogenase, http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Phenylalanine, http://linkedlifedata.com/resource/pubmed/chemical/Prephenate Dehydratase, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Bacterial, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Untranslated, http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Transketolase, http://linkedlifedata.com/resource/pubmed/chemical/YhdA protein, E coli
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0175-7598
pubmed:author
pubmed:issnType
Print
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
283-91
pubmed:dateRevised
2011-10-7
pubmed:meshHeading
pubmed-meshheading:18080813-3-Deoxy-7-Phosphoheptulonate Synthase, pubmed-meshheading:18080813-Chorismate Mutase, pubmed-meshheading:18080813-Escherichia coli, pubmed-meshheading:18080813-Escherichia coli Proteins, pubmed-meshheading:18080813-Gene Deletion, pubmed-meshheading:18080813-Gene Expression Profiling, pubmed-meshheading:18080813-Gene Expression Regulation, Bacterial, pubmed-meshheading:18080813-Glucosephosphate Dehydrogenase, pubmed-meshheading:18080813-Membrane Proteins, pubmed-meshheading:18080813-Metabolic Networks and Pathways, pubmed-meshheading:18080813-Models, Biological, pubmed-meshheading:18080813-Mutagenesis, Insertional, pubmed-meshheading:18080813-Phenylalanine, pubmed-meshheading:18080813-Prephenate Dehydratase, pubmed-meshheading:18080813-RNA, Bacterial, pubmed-meshheading:18080813-RNA, Messenger, pubmed-meshheading:18080813-RNA, Untranslated, pubmed-meshheading:18080813-RNA-Binding Proteins, pubmed-meshheading:18080813-Repressor Proteins, pubmed-meshheading:18080813-Transketolase
pubmed:year
2008
pubmed:articleTitle
Metabolic engineering of Escherichia coli to enhance phenylalanine production.
pubmed:affiliation
Kane Biotech Inc., 5-1250 Waverley Street, Winnipeg, MB, Canada, R3T 6C6.
pubmed:publicationType
Journal Article