Linked Life Data

18078801

Source:http://linkedlifedata.com/resource/pubmed/id/18078801

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2008-3-4
pubmed:abstractText
A TaqMan quantitative real-time PCR detection system was developed to examine transgene copy number in cotton. GhUBC1, a gene validated to be present as a single copy per haploid Gossypium hirsutum genome, was used as the endogenous reference to estimate copy number of GFP and selection marker NPTII in 28 T0 plants. This system was found to be more accurate than genomic Southern blot hybridization and could effectively tell homozygotes from heterozygotes in a T1 transgenic cotton population. Therefore it is suitable for efficient and cost effective early screening of transgenic seedlings and identifying transgene homozygotes in segregation populations.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0003-2697
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
375
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
150-2
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Quantitative real-time PCR assay to detect transgene copy number in cotton (Gossypium hirsutum).
pubmed:affiliation
Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117604, Singapore.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't