Linked Life Data

18078744

Source:http://linkedlifedata.com/resource/pubmed/id/18078744

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2008-2-4
pubmed:abstractText
Gold nanoshells (GNSs) were self-assembled on the surface of transparent glasses modified with 3-aminopropyltrimethoxysilane (APTES) to form GNS self-assembled monolayers (SAMs). Because the localized surface plasmon resonance (LSPR) of GNSs can be controlled in the near-infrared (NIR) region of the spectrum, where the optical transmission through tissue and whole blood is optimal, GNSs would be used as an effective signal transduction in whole blood. Accordingly, after modified with cystamine and biotin-NHS (N-hydroxy succinimide), GNS SAMs were used as a novel optical biosensor for real-time detection of streptavidin-biotin interactions in diluted human whole blood within short assay time, without any sample purification/separation. An UV-vis-NIR spectrophotometer was used to monitor the absorbance changes at 730 nm as a function of time for different concentrations of streptavidin in 20% whole blood, and the results showed that the biosensor displayed low detection limit of approximately 3 microg/mL and wide dynamic range of approximately 3-50 microg/mL. This approach provides an opportunity to construct LSPR biosensor for protein sensing and cellular analysis in diluted whole blood.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0956-5663
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1166-70
pubmed:dateRevised
2009-7-14
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
A label-free biosensor based on gold nanoshell monolayers for monitoring biomolecular interactions in diluted whole blood.
pubmed:affiliation
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Evaluation Studies