Linked Life Data

18075316

Source:http://linkedlifedata.com/resource/pubmed/id/18075316

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2008-2-18
pubmed:abstractText
Disruptions in the expression of the BDNF gene that encodes a neurotrophic factor involved in neuronal survival, differentiation and synaptic plasticity has been proposed to contribute to the molecular pathogenesis of Rett syndrome. Rett syndrome (RTT) is a neurodevelopmental disorder, caused by mutations in the X-linked methyl CpG binding protein 2 gene (MeCP2). MeCP2 deficiency in the brain has been shown to decrease overall expression of BDNF in spite of an observed increase in the activity of promoter III that appears to be controlled directly by MeCP2. Therefore, how MeCP2 deficiency causes an overall downregulation of BDNF expression was an enigma. Here we report that MeCP2 deficiency in human and mouse brain causes an increase in expression of two neuronal gene transcriptional repressors REST (RE1 silencing transcription factor), and CoREST. MeCP2 binds to and is involved in repression of Rest and CoRest promoters despite their unmethylated state. MeCP2 depletion is associated with a change in the histone modification profile to a more active conformation. The elevated levels of REST and CoREST in the brain of RTT patients and MeCP2 deficient mice result in downregulation of BDNF, apparently by their binding to the RE1 (element) located between the first two promoters of the BDNF gene. Interestingly, the NTRK2 gene that encodes the BDNF receptor, TRKB, was overexpressed in MeCP2 deficient human and mouse brains either directly or as an attempt to compensate for BDNF deficiency.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1559-2308
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
214-22
pubmed:dateRevised
2010-1-15
pubmed:meshHeading
pubmed-meshheading:18075316-Animals, pubmed-meshheading:18075316-Base Sequence, pubmed-meshheading:18075316-Brain, pubmed-meshheading:18075316-Brain-Derived Neurotrophic Factor, pubmed-meshheading:18075316-Chromatin Immunoprecipitation, pubmed-meshheading:18075316-DNA Methylation, pubmed-meshheading:18075316-DNA Primers, pubmed-meshheading:18075316-DNA-Binding Proteins, pubmed-meshheading:18075316-Female, pubmed-meshheading:18075316-Humans, pubmed-meshheading:18075316-Male, pubmed-meshheading:18075316-Methyl-CpG-Binding Protein 2, pubmed-meshheading:18075316-Mice, pubmed-meshheading:18075316-Mice, Inbred C57BL, pubmed-meshheading:18075316-Nerve Tissue Proteins, pubmed-meshheading:18075316-Promoter Regions, Genetic, pubmed-meshheading:18075316-Receptor, trkB, pubmed-meshheading:18075316-Repressor Proteins, pubmed-meshheading:18075316-Reverse Transcriptase Polymerase Chain Reaction
pubmed:articleTitle
MeCP2 deficiency in the brain decreases BDNF levels by REST/CoREST-mediated repression and increases TRKB production.
pubmed:affiliation
Department of Cellular Biochemistry and Human Genetics, The Hebrew University, Hadassah Medical School, Jerusalem, Israel.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't