Linked Life Data

18070419

Source:http://linkedlifedata.com/resource/pubmed/id/18070419

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2007-12-11
pubmed:abstractText
The aim of our study was to examine in vivo and in vitro cytokines produced by Lewis ratderived R5-28 sarcoma cells. These cells produce rapidly growing tumours in approximately two weeks after subcutaneous inoculation. However, spontaneous tumour regression was noted in about 40% of animals. For an explanation of this phenomenon, we evaluated the profile of 19 cytokines during tumour growth and spontaneous regression by the use of "antibody array". To detect cytokines directly originated by the sarcoma, the R5-28 cells were cultivated in vitro and then both the supernatants and the cell lysates were analysed. Our experiments showed three cytokines (MCP-1, TIMP-1 and VEGF) to be produced by R5-28 cells in vitro. Moreover, in vivo, another three cytokines (TNF-alpha, beta-NGF and LIX) were detected both in blood sera and tumour lysates, probably produced by immune and stromal cells during tumour growth. Changes in their expression after spontaneous regression are discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0015-5500
pubmed:author
pubmed:issnType
Print
pubmed:volume
53
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
216-9
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Multiplex analysis of cytokines involved in tumour growth and spontaneous regression in a rat sarcoma model.
pubmed:affiliation
Laboratory of Tumour Biology, Institute of Animal Physiology and Genetics, v. v. i., Academy of Sciences of the Czech Republic, Libechov, Czech Republic. neoplasma_9@post.sk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't