Source:http://linkedlifedata.com/resource/pubmed/id/18069249
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
9-10
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pubmed:dateCreated |
2007-12-11
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pubmed:abstractText |
Anther cultures have been developed from transgenic donor peppers carrying HPT/J1-1. Eight out of sixteen plants produced from an anther culture set pepper fruits. Southern blot analysis of donors revealed two independent plants with a single copy of the integrated transgene. PCR and RT-PCR results showed the inheritance of HPT/J1-1 and expression of J1-1 in A1. All A1 progeny derived from transgenic anthers had resistance to hygromycin. They grew normally and showed similar phenotypes to the wild-type. Therefore, the use of an anther culture system coupled with genetic transformation in breeding programs will greatly facilitate the genetic improvement of pepper plants.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0939-5075
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
62
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
743-6
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pubmed:dateRevised |
2009-11-4
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pubmed:meshHeading |
pubmed-meshheading:18069249-Capsicum,
pubmed-meshheading:18069249-Cinnamates,
pubmed-meshheading:18069249-Crosses, Genetic,
pubmed-meshheading:18069249-DNA, Plant,
pubmed-meshheading:18069249-Drug Resistance,
pubmed-meshheading:18069249-Hygromycin B,
pubmed-meshheading:18069249-Plants, Genetically Modified,
pubmed-meshheading:18069249-Polymerase Chain Reaction,
pubmed-meshheading:18069249-Reproduction,
pubmed-meshheading:18069249-Reverse Transcriptase Polymerase Chain Reaction
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pubmed:articleTitle |
Inheritance and expression of transgenes through anther culture of transgenic hot pepper.
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pubmed:affiliation |
Kumho Life and Environmental Science Laboratory & Agricultural Plant Stress Research Center, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju 500-757, Republic of Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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