Linked Life Data

18069019

Source:http://linkedlifedata.com/resource/pubmed/id/18069019

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2008-2-29
pubmed:abstractText
The strategy of mesenchymal stem cells (MSCs) transplantation is limited by the inability to deliver a large number of grafted cells that resist peri-transplantation apoptosis in ischemic tissues, and this led us to investigate methods of improving the viability of these cells. We demonstrate the presence of voltage-gated potassium channels in rat MSCs that can be activated by staurosporine (ST). MSCs exposed to ST underwent apoptotic cell changes. Tetraethylammonium (TEA), a classic blocker of K+ channels, blocked the ST-induced augmentation of K+ currents, and reduced ST-induced apoptosis. Furthermore, we found that TEA prevented the ST-induced increase in expression of the pro-apoptotic protein Bax and decrease of the anti-apoptotic protein Bcl-2. Taken together, our findings suggest that voltage-gated potassium is involved in ST-induced apoptosis of rat MSCs. TEA blocks the ST-induced augmentation of K+ currents, alters the expression of Bcl-2 family proteins induced by ST, and attenuates the apoptosis of rat MSCs.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1065-6995
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
312-9
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Voltage-dependent potassium channels are involved in staurosporine-induced apoptosis of rat mesenchymal stem cells.
pubmed:affiliation
Division of Cardiology, the 2nd Affiliated Hospital, College of Medicine, Zhejiang University, 88 Jie Fang Street, Hangzhou 310009, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't