Source:http://linkedlifedata.com/resource/pubmed/id/18065773
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2008-2-7
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| pubmed:abstractText |
The aim of this study was to investigate whether 4-tert-octylphenol (OP) affects the differentiation of multipotent C3H10T1/2 cells, a cell line established from mouse embryonic connective tissue, into osteoblast and adipocyte lineages. Confluent C3H10T1/2 cells were incubated for 7 days with (OP-treated cultures) or without (control cultures) 15 microg/ml of OP. The 7-day treatment of confluent cells with OP decreased alkaline phosphatase activity by 81%, inhibited the expression of transforming growth factor beta2, and inhibited the morphological changes in cells to an osteoblastic appearance. These results indicate that the 7-day treatment of confluent C3H10T1/2 cells with OP inhibited their differentiation into osteoblasts. Since this treatment strongly induced the expression of peroxisome proliferator-activated receptor r (PPARr) but did not stimulate triacylglycerol (TG) accumulation in cells, C3H10T1/2 cells in the control and OP-treated cultures were incubated for 2 days with a hormone mixture (insulin [INS], dexamethasone, and 1-methyl-3-isobutylxanthine) and incubated for an additional 5 days with INS alone. The TG and adiponectin contents of the OP-treated cultures were 4.2 and 4.1 times higher, respectively, than those of the control cultures. There were many more Oil Red O-staining cells in the OP-treated cultures than in the control cultures. The expression of PPARr in the OP-treated cultures was higher than that in the control cultures. These results indicate that the OP-treated cultures contained a larger number of adipocytes than the control cultures. In conclusion, treatment of C3H10T1/2 cells with OP inhibited osteoblast differentiation, causing a lineage shift toward adipocytes.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2,2-bis(4-glycidyloxyphenyl)propane,
http://linkedlifedata.com/resource/pubmed/chemical/4-nonylphenol,
http://linkedlifedata.com/resource/pubmed/chemical/4-tert-octylphenol,
http://linkedlifedata.com/resource/pubmed/chemical/Adiponectin,
http://linkedlifedata.com/resource/pubmed/chemical/Alkaline Phosphatase,
http://linkedlifedata.com/resource/pubmed/chemical/Epoxy Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Peroxisome Proliferator-Activated...,
http://linkedlifedata.com/resource/pubmed/chemical/Phenols,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta2,
http://linkedlifedata.com/resource/pubmed/chemical/Triglycerides,
http://linkedlifedata.com/resource/pubmed/chemical/Water Pollutants, Chemical,
http://linkedlifedata.com/resource/pubmed/chemical/adiponectin, mouse
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
1096-6080
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
102
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
82-8
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| pubmed:dateRevised |
2010-9-17
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| pubmed:meshHeading |
pubmed-meshheading:18065773-Adipocytes,
pubmed-meshheading:18065773-Adiponectin,
pubmed-meshheading:18065773-Alkaline Phosphatase,
pubmed-meshheading:18065773-Animals,
pubmed-meshheading:18065773-Cell Differentiation,
pubmed-meshheading:18065773-Cell Line,
pubmed-meshheading:18065773-Cell Lineage,
pubmed-meshheading:18065773-Cell Shape,
pubmed-meshheading:18065773-Dose-Response Relationship, Drug,
pubmed-meshheading:18065773-Embryonic Stem Cells,
pubmed-meshheading:18065773-Epoxy Compounds,
pubmed-meshheading:18065773-Mice,
pubmed-meshheading:18065773-Mice, Inbred C3H,
pubmed-meshheading:18065773-Multipotent Stem Cells,
pubmed-meshheading:18065773-Osteoblasts,
pubmed-meshheading:18065773-Peroxisome Proliferator-Activated Receptors,
pubmed-meshheading:18065773-Phenols,
pubmed-meshheading:18065773-Time Factors,
pubmed-meshheading:18065773-Transforming Growth Factor beta2,
pubmed-meshheading:18065773-Triglycerides,
pubmed-meshheading:18065773-Water Pollutants, Chemical
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| pubmed:year |
2008
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| pubmed:articleTitle |
4-tert-octylphenol regulates the differentiation of C3H10T1/2 cells into osteoblast and adipocyte lineages.
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| pubmed:affiliation |
Department of Bone and Joint Surgery, Ehime University Graduate School of Medicine, Toon, Ehime 791-0295, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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