|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2008-1-24
|
|
pubmed:abstractText |
Twist is a basic helix loop helix protein that plays a role both in human development and in cancer biogenesis. While characterizing the effects of Twist on breast epithelial cell transformation, we identified E-cadherin as a target gene that is down-regulated by Twist. In this study, we demonstrate that Twist can transcriptionally repress E-cadherin in breast cancer cells. Using transient promoter assays, we show that Twist can down-regulate E-cadherin promoter activity by up to two folds. This is further supported by immunoblot analyses which indicates that over-expression of Twist decreases E-cadherin protein levels in breast cancer cell lines. Subsequently, chromatin immunoprecipitation performed on MCF-7/Twist and Hs578 T (high level of endogenous Twist expression) confirmed Twist binding to the E-cadherin promoter. Finally, the functional relevance of this regulation was verified by quantitative real-time PCR and immunohistochemistry on a cohort of breast cancer samples.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18062917-10572240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18062917-10655587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18062917-11912130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18062917-12217327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18062917-12414534,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18062917-15950916,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18062917-15958559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18062917-16116478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18062917-16322226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18062917-16756717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18062917-2493990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18062917-3106932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18062917-7543680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18062917-7729687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18062917-8378299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18062917-8459805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18062917-8557030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18062917-8608588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18062917-8988167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18062917-9073070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18062917-9218871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18062917-9343420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18062917-9708792
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
1090-2104
|
|
pubmed:author |
|
|
pubmed:issnType |
Electronic
|
|
pubmed:day |
7
|
|
pubmed:volume |
367
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
235-41
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:18062917-Breast Neoplasms,
pubmed-meshheading:18062917-Cadherins,
pubmed-meshheading:18062917-Cell Line, Tumor,
pubmed-meshheading:18062917-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:18062917-Humans,
pubmed-meshheading:18062917-Nuclear Proteins,
pubmed-meshheading:18062917-Repressor Proteins,
pubmed-meshheading:18062917-Transcriptional Activation,
pubmed-meshheading:18062917-Twist Transcription Factor
|
|
pubmed:year |
2008
|
|
pubmed:articleTitle |
Twist is a transcriptional repressor of E-cadherin gene expression in breast cancer.
|
|
pubmed:affiliation |
Department of Radiology, Johns Hopkins University School of Medicine, 340 Traylor , 720 Rutland Avenue, Baltimore, MD 21205, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
|
