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pubmed-article:18061476pubmed:abstractTextIsobaric oligosaccharides enzymatically prepared from hyaluronic acid (HA) and N-acetylheparosan (NAH), were distinguished using tandem mass spectrometry. The only difference between the two series of oligosaccharides was the linkage pattern (in HA 1-->3 and in NAH 1-->4) between glucuronic acid and N-acetylglucosamine residues. Tandem mass spectrometry afforded spectra in which glycosidic cleavage fragment ions were observed for both HA and NAH oligosaccharides. Cross-ring cleavage ions 0,2An and 0,2An-h (n is even number) were observed only in GlcNAc residues of NAH oligosaccharides. One exception was an 0,2A2 ion fragment observed for the disaccharide from HA. These cross-ring cleavage fragment ions are useful to definitively distinguish HA and NAH oligosaccharides.lld:pubmed
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pubmed-article:18061476pubmed:authorpubmed-author:LiuJianJlld:pubmed
pubmed-article:18061476pubmed:authorpubmed-author:LinhardtRober...lld:pubmed
pubmed-article:18061476pubmed:authorpubmed-author:TUIP CPClld:pubmed
pubmed-article:18061476pubmed:authorpubmed-author:ZhangZhenqing...lld:pubmed
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pubmed-article:18061476pubmed:dateRevised2011-8-1lld:pubmed
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pubmed-article:18061476pubmed:articleTitleTandem MS can distinguish hyaluronic acid from N-acetylheparosan.lld:pubmed
pubmed-article:18061476pubmed:affiliationDepartment of Chemistry, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.lld:pubmed
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pubmed-article:18061476pubmed:publicationTypeResearch Support, N.I.H., Extramurallld:pubmed
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