Linked Life Data

1806027

Source:http://linkedlifedata.com/resource/pubmed/id/1806027

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1992-5-8
pubmed:abstractText
Using BamHI digested and dephosphorylated pBR322 as vector, a GL-7-ACA acylase gene from Pseudomonas sp. 130 chromosomal DNA was cloned and expressed in Escherichia coli C600. Seven positive clones were detected from 3205 recombinant plasmids with 32P-labeled oligonucleotide probes in situ hybridization. Out of them, three clones which produced active GL-7-ACA acylase were identified by radio-immunological assay, chemical test and chromatographic analysis of reaction mixture. The plasmid DNAs of recombinant pMR5, pMR6 and pMR7 were extracted. Analysis of gel electrophoresis indicated that pMR5 and pMR7 contained the same 6.8kb fragment and the size of the insert in pNR6 was 5.7 kb. The effects of various E. coli hosts on the expression of cloned GL-7-ACA acylase gene is also presented.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1042-749X
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
93-104
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Cloning of GL-7-ACA acylase gene from Pseudomonas sp. 130 and its expression in Escherichia coli.
pubmed:affiliation
Shanghai Institute of Plant Physiology, Chinese Academy of Sciences.
pubmed:publicationType
Journal Article, Comparative Study