Linked Life Data

1805966

Source:http://linkedlifedata.com/resource/pubmed/id/1805966

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1992-5-13
pubmed:abstractText
Mutations in the prfB gene which encodes peptide-chain-release factor 2 of Escherichia coli were defined by DNA sequence analysis. prfB1 and prfB3 substitute lysine and asparagine for glutamate and aspartate at amino acid positions 89 and 143, respectively. Temperature-sensitive mutations, prfB2 and prfB286, each contain the identical substitution of phenylalanine for leucine-328. These mutations suppress UGA but not UAG or UAA. The efficiency of suppression was affected by the neighboring RNA context. The prfB gene encodes a premature UGA stop codon at position 26 and is expressed by +1 frameshifting. The efficiency of natural frameshift was 18% as measured by using the monolysogenic lambda assay vector containing prfB-lacZ fusions, and increased up to 30% in the prfB mutants. These observations can be interpreted as genetic evidence for the autogenous control of RF2 synthesis by frameshifting. Structural and functional organizations of release factors are discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0300-9084
pubmed:author
pubmed:issnType
Print
pubmed:volume
73
pubmed:geneSymbol
prfB
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1509-16
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Sequence and functional analysis of mutations in the gene encoding peptide-chain-release factor 2 of Escherichia coli.
pubmed:affiliation
Department of Tumor Biology, University of Tokyo, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't