Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2007-12-6
pubmed:abstractText
Muscle atrophy occurs in many pathological states and results primarily from accelerated protein degradation and activation of the ubiquitin-proteasome pathway. However, the importance of lysosomes in muscle atrophy has received little attention. Activation of FoxO transcription factors is essential for the atrophy induced by denervation or fasting, and activated FoxO3 by itself causes marked atrophy of muscles and myotubes. Here, we report that FoxO3 does so by stimulating overall protein degradation and coordinately activating both lysosomal and proteasomal pathways. Surprisingly, in C2C12 myotubes, most of this increased proteolysis is mediated by lysosomes. Activated FoxO3 stimulates lysosomal proteolysis in muscle (and other cell types) by activating autophagy. FoxO3 also induces the expression of many autophagy-related genes, which are induced similarly in mouse muscles atrophying due to denervation or fasting. These studies indicate that decreased IGF-1-PI3K-Akt signaling activates autophagy not only through mTOR but also more slowly by a transcription-dependent mechanism involving FoxO3.
pubmed:grant
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1550-4131
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
472-83
pubmed:dateRevised
2010-12-3
pubmed:meshHeading
pubmed-meshheading:18054316-Animals, pubmed-meshheading:18054316-Autophagy, pubmed-meshheading:18054316-Base Sequence, pubmed-meshheading:18054316-Cell Line, pubmed-meshheading:18054316-DNA, pubmed-meshheading:18054316-Forkhead Transcription Factors, pubmed-meshheading:18054316-Gene Expression Regulation, pubmed-meshheading:18054316-Lysosomes, pubmed-meshheading:18054316-Mice, pubmed-meshheading:18054316-Mice, Knockout, pubmed-meshheading:18054316-Models, Biological, pubmed-meshheading:18054316-Muscle Fibers, Skeletal, pubmed-meshheading:18054316-Muscle Proteins, pubmed-meshheading:18054316-Muscular Atrophy, pubmed-meshheading:18054316-Phosphatidylinositol 3-Kinases, pubmed-meshheading:18054316-Proteasome Endopeptidase Complex, pubmed-meshheading:18054316-Proto-Oncogene Proteins c-akt, pubmed-meshheading:18054316-SKP Cullin F-Box Protein Ligases, pubmed-meshheading:18054316-Ubiquitin-Protein Ligases
pubmed:year
2007
pubmed:articleTitle
FoxO3 coordinately activates protein degradation by the autophagic/lysosomal and proteasomal pathways in atrophying muscle cells.
pubmed:affiliation
Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural