Linked Life Data

18053803

Source:http://linkedlifedata.com/resource/pubmed/id/18053803

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2007-12-25
pubmed:abstractText
The sulfate-reducing strain AK-01 activates alkanes via addition of the subterminal carbon to the double bond of fumarate. This reaction is similar to the action of the glycyl radical enzyme benzylsuccinate synthase (Bss). It was hypothesized that strain AK-01 possesses a similar enzyme. Degenerate bssA primers and inverse PCR were used to amplify two unlinked genes (assA1 and assA2), which encode catalytic subunits of glycyl radical type enzymes. Subsequent genome sequencing of AK-01 revealed two ass operons. SDS-PAGE analysis of AK-01 grown on n-hexadecane revealed a 95-kDa protein which is absent in hexadecanoate-grown cells. LC-MS/MS data obtained from a tryptic digest of this protein match the deduced amino acid sequence encoded by assA1, thus confirming AssA1's involvement in alkane metabolism. This report is the first description of a gene involved in anaerobic n-alkane metabolism in a sulfate-reducer and provides evidence for a novel glycyl radical enzyme.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1090-2104
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
366
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
142-8
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Anaerobic alkane-degrading strain AK-01 contains two alkylsuccinate synthase genes.
pubmed:affiliation
Biotechnology Center for Agriculture and the Environment, School of Environmental and Biological Sciences, Rutgers University, New Brunswick, NJ 08901-8520, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, N.I.H., Extramural