|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
12
|
|
pubmed:dateCreated |
2007-11-29
|
|
pubmed:abstractText |
The photophysical, electrochemical and spectroscopic characteristics of a conjugate of 3-devinyl-3-(1'-hexyloxyethyl)pyropheophorbide-a (HPPH) and a cyanine dye have been investigated both as a linked conjugate and as individual components. A photoexcitation of the HPPH moiety of the conjugate results in electron transfer from the singlet excited state of HPPH (1HPPH*) to the cyanine dye as well as that from the cyanine dye to 1HPPH* and is followed in both cases by facile back electron transfer to the ground state as indicated by time-resolved fluorescence and transient absorption measurements. Intersystem crossing to the triplet excited state (3HPPH*) competes with the electron transfer and 3HPPH* is quenched by oxygen to produce singlet oxygen (1O2), leading to specific covalent cross-linking of the nonactivated signal transducer and activator of transcription (STAT-3). In contrast to excitation of the HPPH moiety, photoexcitation of the cyanine dye unit results in a strong emission at 875 nm, which can be used for efficient tumor imaging. Compared to HPPH alone, the presence of the cyanine dye moiety in the conjugate produces a significantly higher uptake in tumors than in skin. Thus, the HPPH-cyanine dye conjugate can be used as a dual tumor imaging and photodynamic therapy agent.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
1474-905X
|
|
pubmed:author |
pubmed-author:BaumannHeinzH,
pubmed-author:ChenYihuiY,
pubmed-author:CiesielskiMichaelM,
pubmed-author:FenstermakerRobertR,
pubmed-author:FukuzumiShunichiS,
pubmed-author:KadishKarl MKM,
pubmed-author:LiuWeiguoW,
pubmed-author:OhkuboKeiK,
pubmed-author:OseroffAllanA,
pubmed-author:PandeyRavindra KRK,
pubmed-author:PandeySuresh KSK,
pubmed-author:TracyErinE,
pubmed-author:WenboEE,
pubmed-author:ZhangMinM
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
6
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1257-67
|
|
pubmed:meshHeading |
pubmed-meshheading:18046480-Animals,
pubmed-meshheading:18046480-Carbocyanines,
pubmed-meshheading:18046480-Chlorophyll,
pubmed-meshheading:18046480-Cross-Linking Reagents,
pubmed-meshheading:18046480-Electrochemistry,
pubmed-meshheading:18046480-Fluorescence,
pubmed-meshheading:18046480-Glioma,
pubmed-meshheading:18046480-Mice,
pubmed-meshheading:18046480-Mice, Nude,
pubmed-meshheading:18046480-Molecular Structure,
pubmed-meshheading:18046480-Neoplasm Transplantation,
pubmed-meshheading:18046480-Oxidation-Reduction,
pubmed-meshheading:18046480-Photochemotherapy,
pubmed-meshheading:18046480-Photosensitizing Agents,
pubmed-meshheading:18046480-STAT3 Transcription Factor,
pubmed-meshheading:18046480-Singlet Oxygen
|
|
pubmed:year |
2007
|
|
pubmed:articleTitle |
Photophysical, electrochemical characteristics and cross-linking of STAT-3 protein by an efficient bifunctional agent for fluorescence image-guided photodynamic therapy.
|
|
pubmed:affiliation |
Photodynamic Therapy Center, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|
