Linked Life Data

18041742

Source:http://linkedlifedata.com/resource/pubmed/id/18041742

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2008-2-4
pubmed:abstractText
NY-BR-1 is a differentiation antigen and a potential target for cancer immunotherapy. Its mRNA expression is restricted to breast, testis, prostate and breast cancer by RT-PCR. In this study, we correlated NY-BR-1 protein and mRNA expression on tissue microarrays of mammary, prostatic and testicular malignancies using immunohistochemistry and in situ hybridization with probes for exon 4-7 and 30-33. NY-BR-1 mRNA was confined to primary spermatocytes, suggesting a role in spermatogenesis. Exon 4-7 and 30-33 were equally expressed this cell type. However, NY-BR-1 was absent in all germ cell tumours analyzed (n = 475) and present in one of 56 (2%) prostate carcinomas. In breast, NY-BR-1 mRNA expression was detected in 307 of 442 (70%) primary carcinomas, with strong correlation to its protein expression (p < 0.0001). mRNA expression was significantly stronger and more frequently detected by the exon 30-33 probe than by the exon 4-7 probe (70% vs. 35%, p < 0.0001), indicating the presence of alternative splice variants that lack 5-prime sequences. A similar restricted mRNA pattern was also observed in the normal breast epithelium. NY-BR-1 protein and mRNA correlated significantly with estrogen receptor alpha (ER alpha) protein expression (p < 0.0001), with stronger association to NY-BR-1 mRNA than protein (odds ratio 7.7 compared to 4.6). We identified 4 estrogen response elements (ERE)-like sequences nearby the promoter region, suggesting that NY-BR-1 transcription might be controlled by ER alpha. Accordingly, analysis of matching pairs of primary tumors with their recurrences showed a marked decrease of NY-BR-1 expression in recurrences after tamoxifen treatment (p < 0.0001).
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1097-0215
pubmed:author
pubmed:copyrightInfo
(c) 2007 Wiley-Liss, Inc.
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
122
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1585-91
pubmed:meshHeading
pubmed-meshheading:18041742-Antigens, Neoplasm, pubmed-meshheading:18041742-Antineoplastic Agents, Hormonal, pubmed-meshheading:18041742-Breast, pubmed-meshheading:18041742-Breast Neoplasms, pubmed-meshheading:18041742-Estrogen Receptor Modulators, pubmed-meshheading:18041742-Female, pubmed-meshheading:18041742-Gene Expression Regulation, Neoplastic, pubmed-meshheading:18041742-Humans, pubmed-meshheading:18041742-Immunohistochemistry, pubmed-meshheading:18041742-In Situ Hybridization, pubmed-meshheading:18041742-Male, pubmed-meshheading:18041742-Prostatic Neoplasms, pubmed-meshheading:18041742-RNA, Messenger, pubmed-meshheading:18041742-Receptors, Estrogen, pubmed-meshheading:18041742-Response Elements, pubmed-meshheading:18041742-Tamoxifen, pubmed-meshheading:18041742-Testicular Neoplasms, pubmed-meshheading:18041742-Testis, pubmed-meshheading:18041742-Tumor Markers, Biological
pubmed:year
2008
pubmed:articleTitle
Distinct expression patterns of the immunogenic differentiation antigen NY-BR-1 in normal breast, testis and their malignant counterparts.
pubmed:affiliation
Institute of Surgical Pathology, Department Pathology, University Hospital of Zürich, Switzerland. jean-philippe.theurillat@cell.biol.ethz.ch
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural