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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2007-11-27
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pubmed:abstractText |
The Drosophila dec-1 gene produces three proproteins required for female fertility and eggshell assembly. The three proproteins are distinguished by their C termini. Fc106, the most abundant proprotein, is cleaved within the vitelline membrane to three mature derivatives in a developmentally regulated manner. To define sequences within fc106 that are critical for its function, we created wild-type and mutant versions of an fc106 cDNA transgene. The functional consequences of the mutations were assessed in dec-14, a female-sterile splicing mutant that does not produce the fc106 isoform. The fertility of dec-14 females was restored by the introduction of either a wild-type transgene or a transgene bearing a C-terminal deletion that included fc106-specific sequences. Surprisingly, the removal of internal coding sequences created an aberrant DEC-1 proprotein that induced female sterility when introduced into wild-type flies. Dominant female sterility was not associated with larger deletions that included the fc106 N terminus, suggesting that abnormal juxtaposition of N- and C-terminal sequences in the aberrant proprotein interfered with endogenous DEC-1 proteins. Changes in the fractionation behavior of the endogenous fc106 C-terminal derivative, s60, and morphological changes in the endochorion in response to expression of the aberrant proprotein support this interpretation.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18039879-10804461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18039879-10985863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18039879-110454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18039879-11729155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18039879-12052430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18039879-12648483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18039879-12651868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18039879-12840783,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18039879-13678954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18039879-14871609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18039879-15136154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18039879-15563506,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18039879-1699826,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18039879-17246182,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18039879-2504634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18039879-3108048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18039879-3378704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18039879-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18039879-7589807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18039879-8806834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18039879-8825147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18039879-9535559
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0016-6731
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
177
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1595-608
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pubmed:dateRevised |
2010-9-16
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pubmed:meshHeading |
pubmed-meshheading:18039879-Amino Acid Sequence,
pubmed-meshheading:18039879-Animals,
pubmed-meshheading:18039879-Animals, Genetically Modified,
pubmed-meshheading:18039879-Base Sequence,
pubmed-meshheading:18039879-DNA Primers,
pubmed-meshheading:18039879-Drosophila Proteins,
pubmed-meshheading:18039879-Drosophila melanogaster,
pubmed-meshheading:18039879-Egg Proteins,
pubmed-meshheading:18039879-Female,
pubmed-meshheading:18039879-Genes, Dominant,
pubmed-meshheading:18039879-Genes, Insect,
pubmed-meshheading:18039879-Infertility, Female,
pubmed-meshheading:18039879-Molecular Sequence Data,
pubmed-meshheading:18039879-Mutation,
pubmed-meshheading:18039879-Ovum,
pubmed-meshheading:18039879-Recombinant Proteins,
pubmed-meshheading:18039879-Sequence Deletion
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pubmed:year |
2007
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pubmed:articleTitle |
A mutant dec-1 transgene induces dominant female sterility in Drosophila melanogaster.
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pubmed:affiliation |
Department of Biological Sciences, Marquette University, Milwaukee, Wisconsin 53233, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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