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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
1992-4-24
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pubmed:abstractText |
The influence of the maintenance medium, polyethylene glycol (PEG), DEAE-dextran, and low temperature on reproduction of influenza A, B, and C viruses and their reassortants in diploid and continuous cell cultures was determined. Lowering of pH in the maintenance medium to 6.5 was found to decrease reproduction of influenza A (H1N1) and A (H3N2) viruses and increase that of influenza B viruses. Treatment of cells with PEG solution increased the yield of influenza B and C but not A viruses. However, influenza A virus strains proved to be capable of producing infectious progeny in nonpermissive cell lines treated with PEG. Addition of DEAE-dextran to the medium exerted no effect on the infectivity of influenza A and B reassortants. Moreover, infection of MDCK cells after a "cold shock" led to an increase in hemagglutinin titres in influenza A reassortants.
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pubmed:language |
rus
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0507-4088
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
36
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
384-6
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:1803770-Animals,
pubmed-meshheading:1803770-Cells, Cultured,
pubmed-meshheading:1803770-Chemistry, Physical,
pubmed-meshheading:1803770-Culture Media,
pubmed-meshheading:1803770-DEAE-Dextran,
pubmed-meshheading:1803770-Humans,
pubmed-meshheading:1803770-Hydrogen-Ion Concentration,
pubmed-meshheading:1803770-Influenza A virus,
pubmed-meshheading:1803770-Influenza B virus,
pubmed-meshheading:1803770-Influenzavirus C,
pubmed-meshheading:1803770-Physicochemical Phenomena,
pubmed-meshheading:1803770-Polyethylene Glycols,
pubmed-meshheading:1803770-Reassortant Viruses,
pubmed-meshheading:1803770-Temperature,
pubmed-meshheading:1803770-Virus Cultivation,
pubmed-meshheading:1803770-Virus Replication
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pubmed:articleTitle |
[The effect of different factors on the reproduction of influenza viruses and reassortants in cell cultures].
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pubmed:publicationType |
Journal Article,
English Abstract
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