Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2007-11-23
pubmed:abstractText
This study investigated the antimicrobial activities of chitosan-lysozyme (CL) composite films and coatings against tested microorganisms inoculated onto the surface of Mozzarella cheese. CL film-forming solutions (FFS) with a pH of 4.4 to 4.5 were prepared by incorporating 0% or 60% lysozyme (per dry weight of chitosan) into chitosan FFS with or without a pH adjustment to 5.2. Sliced cheese was subjected to 3 CL package applications: film, lamination on a multilayer coextruded film, and coating. Cheese was inoculated with Listeria monocytogenes, Escherichia coli, or Pseudomonas fluorescens at 10(4) CFU/g, or with mold and yeast at 10(2) CFU/g. Inoculated cheese was individually vacuum packaged and stored at 10 degrees C for sampling at 1, 7, and 14 d for bacteria, and at 10, 20, and 30 d for fungi. Inoculated bacteria survived but failed to multiply in untreated cheese during storage. Treated cheese received 0.43- to 1.25-, 0.40- to 1.40-, and 0.32- to 1.35-log reductions in E. coli, P. fluorescens, and L. monocytogenes, respectively. Incorporation of 60% lysozyme in chitosan FFS showed greater antimicrobial effect than chitosan alone on P. fluorescens and L. monocytogenes. The pH adjustment only affected the antimicrobial activity on L. monocytogenes, with lower pH (unadjusted) showing greater antimicrobial effect than pH 5.2. Mold and yeast increased to 10(5) CFU/g in untreated cheese after 30 d storage. Growth of mold was completely inhibited in cheese packaged with CL films, while 0.24- to 1.90- and 0.06- to 0.50-log reductions in mold populations were observed in cheese packaged with CL-laminated films and coatings, respectively. All CL packaging applications resulted in 0.01- to 0.64-log reduction in yeast populations.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1750-3841
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
72
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
M355-62
pubmed:meshHeading
pubmed-meshheading:18034728-Analysis of Variance, pubmed-meshheading:18034728-Anti-Infective Agents, pubmed-meshheading:18034728-Bacteria, pubmed-meshheading:18034728-Cheese, pubmed-meshheading:18034728-Chitosan, pubmed-meshheading:18034728-Colony Count, Microbial, pubmed-meshheading:18034728-Escherichia coli, pubmed-meshheading:18034728-Food Contamination, pubmed-meshheading:18034728-Food Handling, pubmed-meshheading:18034728-Food Microbiology, pubmed-meshheading:18034728-Food Packaging, pubmed-meshheading:18034728-Fungi, pubmed-meshheading:18034728-Hydrogen-Ion Concentration, pubmed-meshheading:18034728-Listeria monocytogenes, pubmed-meshheading:18034728-Membranes, Artificial, pubmed-meshheading:18034728-Muramidase, pubmed-meshheading:18034728-Pseudomonas fluorescens, pubmed-meshheading:18034728-Yeasts
pubmed:year
2007
pubmed:articleTitle
Antimicrobial chitosan-lysozyme (CL) films and coatings for enhancing microbial safety of mozzarella cheese.
pubmed:affiliation
Dept. of Food Science and Technology, 100 Wiegand Hall, Oregon State Univ., Corvallis, OR 97331-6602, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't