Linked Life Data

18034703

Source:http://linkedlifedata.com/resource/pubmed/id/18034703

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2007-11-23
pubmed:abstractText
An extreme thermostable and acidic tolerable beta-glucanase was isolated and characterized from aerobic fungi Trichoderma koningii ZJU-T. The optimal reaction temperature and pH for the beta-glucanase were 100 degrees C and pH 2.0, respectively. The beta-glucanase showed increased stability at higher temperatures and lower pH values when compared to other beta-glucanases. The optimum conditions for the beta-glucanase stability were found to be pH 4.0 and 80 degrees C. Even subjected to 100 degrees C for 3 h, beta-glucanase activity did not show significant reduction. Moreover, K(+) significantly enhanced beta-glucanase activity at the concentration of 1 mM, while EDTA and other metal ions such as Mg(2+), Mn(2+), Zn(2+), Ca(2+), Fe(2+), Pb(2+), and Fe(3+) inhibited beta-glucanase activity. Denaturants, including sodium dodecyl sulfate (SDS) and mercaptoethanol, also inhibited beta-glucanase activity at a concentration of 5%. However, in the presence of 7 M urea, residual activity of the beta-glucanase still remained 14.5%.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1750-3841
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
72
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
C452-6
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Characterization of a thermostable and acidic-tolerable beta-glucanase from aerobic fungi Trichoderma koningii ZJU-T.
pubmed:affiliation
Dept. of Food Science and Nutrition, Zhejiang Univ., Hangzhou 310029, P. R. China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't