Linked Life Data

18032387

Source:http://linkedlifedata.com/resource/pubmed/id/18032387

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2008-1-21
pubmed:databankReference
pubmed:abstractText
Adenosine deaminase-related growth factors (ADGF), also known as CECR1 in vertebrates, are a novel family of growth factors with sequence similarity to classical cellular adenosine deaminase. Although genes for ADGF/CECR1 have been identified in both invertebrates as well as vertebrates, their in vivo functions in vertebrates remain unknown. We isolated cDNA clones for two cerc 1s from Xenopus laevis. Both recombinant Xenopus CECR1s exhibited adenosine deaminase and growth factor activity, and the adenosine deaminase activity was found to be indispensable for growth factor activity. The Xenopus cerc 1s are expressed in the somites, pronephros, eyes, cement gland, neural tube, and neural floor plate of the embryos. Knock-down of these two genes using morpholino oligonucleotides caused a reduction in the body size and abnormalities of the body axis in the Xenopus embryos, accompanied by selective changes in the expression of developmental marker genes. Injection of adenosine, agonists for adenosine/P1 receptors, or adenosine deaminase inhibitor into late gastrula archenteron embryos resulted in developmental defects similar to those caused by morpholino oligonucleotide injection. These results show, for the first time, the involvement of CECR1s via the adenosine/P1 receptors in vertebrate embryogenesis via regulation of extracellular adenosine concentrations.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
283
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2255-64
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:18032387-Adenosine, pubmed-meshheading:18032387-Adenosine Deaminase, pubmed-meshheading:18032387-Animals, pubmed-meshheading:18032387-Base Sequence, pubmed-meshheading:18032387-Body Size, pubmed-meshheading:18032387-Embryonic Development, pubmed-meshheading:18032387-Gastrula, pubmed-meshheading:18032387-Gene Expression Regulation, Developmental, pubmed-meshheading:18032387-Intercellular Signaling Peptides and Proteins, pubmed-meshheading:18032387-Molecular Sequence Data, pubmed-meshheading:18032387-Oligonucleotides, Antisense, pubmed-meshheading:18032387-Organ Specificity, pubmed-meshheading:18032387-Purinergic P1 Receptor Agonists, pubmed-meshheading:18032387-Receptors, Purinergic P1, pubmed-meshheading:18032387-Vasodilator Agents, pubmed-meshheading:18032387-Xenopus Proteins, pubmed-meshheading:18032387-Xenopus laevis
pubmed:year
2008
pubmed:articleTitle
The extracellular adenosine deaminase growth factor, ADGF/CECR1, plays a role in Xenopus embryogenesis via the adenosine/P1 receptor.
pubmed:affiliation
Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't