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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2007-11-22
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pubmed:abstractText |
The chemokine CXCL12 induces prolonged focal adhesion kinase (FAK) phosphorylation and sustained proadhesive responses in progenitor bone-marrow (BM) B cells, but not in mature peripheral B cells. Here we demonstrate that suppressor of cytokine signaling 3 (SOCS3) regulated CXCL12-induced FAK phosphorylation through the ubiquitin-proteasome pathway. CXCL12 triggered increased FAK ubiquitination in mature B cells, but not in progenitor B cells. Accordingly, SOCS3 expression was low in progenitor B cells, increased in immature B cells, and highest in mature B cells. SOCS3 overexpression in pro-B cells impaired CXCL12-induced FAK phosphorylation and proadhesive responses. Conversely, SOCS3-deficient mature B cells from Cre(MMTV)Socs3(fl/fl) mice exhibited prolonged FAK phosphorylation and adhesion to VCAM-1. In contrast to wild-type mice, Cre(MMTV)Socs3(fl/fl) mice had a 2-fold increase in immature B cells, which were evenly distributed in endosteal and perisinusoidal BM compartments. We propose that the developmental regulation of CXCR4-FAK signaling by SOCS3 is an important mechanism to control the lodgement of B cell precursors in the BM microenvironment.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CXCR4 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Focal Adhesion Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Ptk2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, CXCR4,
http://linkedlifedata.com/resource/pubmed/chemical/Socs3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Suppressor of Cytokine Signaling...,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Cell Adhesion Molecule-1
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1074-7613
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
27
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
811-23
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:18031698-Animals,
pubmed-meshheading:18031698-B-Lymphocytes,
pubmed-meshheading:18031698-Cell Adhesion,
pubmed-meshheading:18031698-Flow Cytometry,
pubmed-meshheading:18031698-Fluorescent Antibody Technique,
pubmed-meshheading:18031698-Focal Adhesion Kinase 1,
pubmed-meshheading:18031698-Humans,
pubmed-meshheading:18031698-Immunoblotting,
pubmed-meshheading:18031698-Immunohistochemistry,
pubmed-meshheading:18031698-Immunoprecipitation,
pubmed-meshheading:18031698-Lymphopoiesis,
pubmed-meshheading:18031698-Mice,
pubmed-meshheading:18031698-Phosphorylation,
pubmed-meshheading:18031698-Precursor Cells, B-Lymphoid,
pubmed-meshheading:18031698-Receptors, CXCR4,
pubmed-meshheading:18031698-Signal Transduction,
pubmed-meshheading:18031698-Suppressor of Cytokine Signaling Proteins,
pubmed-meshheading:18031698-Transfection,
pubmed-meshheading:18031698-Ubiquitination,
pubmed-meshheading:18031698-Vascular Cell Adhesion Molecule-1
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pubmed:year |
2007
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pubmed:articleTitle |
SOCS3 protein developmentally regulates the chemokine receptor CXCR4-FAK signaling pathway during B lymphopoiesis.
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pubmed:affiliation |
Children's Hospital Boston and Joint Program in Transfusion Medicine, Harvard Medical School, Boston, MA 02115, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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