Source:http://linkedlifedata.com/resource/pubmed/id/18027867
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
2008-1-29
|
| pubmed:abstractText |
In this paper we characterize hPARM-1, the human ortholog of rat PARM-1 (prostatic androgen-repressed message-1) and demonstrate its role in prostate cancer. Immunofluorescence microscopy and ultrastructural analysis revealed the localization of hPARM-1 to Golgi, plasma membrane and the early endocytic pathway but not in lysosomes. Biochemical and deglycosylation studies showed hPARM-1 as a highly glycosylated, mucin-like type I transmembrane protein. Analysis of expression of hPARM-1 in various human tissues revealed its presence in most human tissues with especially high expression in heart, kidney and placenta. Androgen controls the expression of the gene as a marked 7-fold increase is seen in the androgen-dependent prostate cancer cell line, LNCaP on androgen stimulation. This is further supported by its decrease in expression in CWR22 xenograft upon castration. Moreover, ectopic expression of hPARM-1 in PC3 prostate cancer cells increased colony formation, suggesting a probable role in cell proliferation. These results suggest that hPARM-1 may have a role in normal biology of the prostate cell and in prostate cancer.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
1097-0215
|
| pubmed:author | |
| pubmed:copyrightInfo |
(c) 2007 Wiley-Liss, Inc.
|
| pubmed:issnType |
Electronic
|
| pubmed:day |
15
|
| pubmed:volume |
122
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1229-35
|
| pubmed:meshHeading |
pubmed-meshheading:18027867-Amino Acid Sequence,
pubmed-meshheading:18027867-Androgen-Binding Protein,
pubmed-meshheading:18027867-Androgens,
pubmed-meshheading:18027867-Animals,
pubmed-meshheading:18027867-Base Sequence,
pubmed-meshheading:18027867-Cell Line, Tumor,
pubmed-meshheading:18027867-Cell Proliferation,
pubmed-meshheading:18027867-Cryoelectron Microscopy,
pubmed-meshheading:18027867-DNA Primers,
pubmed-meshheading:18027867-Dogs,
pubmed-meshheading:18027867-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:18027867-Glycosylation,
pubmed-meshheading:18027867-Humans,
pubmed-meshheading:18027867-Male,
pubmed-meshheading:18027867-Microscopy, Fluorescence,
pubmed-meshheading:18027867-Molecular Sequence Data,
pubmed-meshheading:18027867-Prostatic Neoplasms,
pubmed-meshheading:18027867-Rats,
pubmed-meshheading:18027867-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:18027867-Sequence Homology, Amino Acid
|
| pubmed:year |
2008
|
| pubmed:articleTitle |
Human PARM-1 is a novel mucin-like, androgen-regulated gene exhibiting proliferative effects in prostate cancer cells.
|
| pubmed:affiliation |
Department of Molecular Biosciences, University of Oslo, Oslo, Norway.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|