Linked Life Data

18026855

Source:http://linkedlifedata.com/resource/pubmed/id/18026855

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2008-1-24
pubmed:abstractText
The study was aimed to investigate the role of the "extracellular matrix (ECM)-integrins-cytoskeleton" signal pathway in mechanotransduction of centrifugal loading. MG-63 osteoblasts were exposed to centrifugal loading at 209xg for 10 min. Uncentrifuged cells and centrifuged cells that have been trypsinized and suspended in liquors were designed as control. The changes in F-actin and alpha-actin cytoskeleton, gene transcription of ECM components, and integrins expression were analyzed by LSCM, Real-Time RT-PCR and FCM, respectively. A temporary and fast reversible change was observed in F-actin and alpha-actin cytoskeleton. And the change was paralleled with the fast autoregulation in gene transcription of ECM components of fibronection, osteopontin and Collagen I, and integrins expression of both alpha2 and beta1 subunits. The result suggested that cytoskeleton was a possible mechanical sensor to centrifugal stimuli, and the cytoskeleton regulation to centrifugal loading was in an ECM-dependent and integrin-mediated manner.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0300-8177
pubmed:author
pubmed:issnType
Print
pubmed:volume
309
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
41-8
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
The role of extracellular matrix, integrins, and cytoskeleton in mechanotransduction of centrifugal loading.
pubmed:affiliation
Department of Orthodontics, West China College of Stomatology, Sichuan University, 14#, 3rd section, Renmin South Road, Chengdu 610041, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't