Linked Life Data

18023408

Source:http://linkedlifedata.com/resource/pubmed/id/18023408

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2008-1-9
pubmed:abstractText
Many biochemical and biophysical methods can be used to characterize the oligomerization state of proteins. One of the most widely used is glutaraldehyde crosslinking, mainly because of the minimum equipment and reagents required. However, the crosslinking procedures currently in use are impaired by the low specificity of the reagent, which can chemically bond any two amino groups that are close in space. Thus, extensive and time-consuming investigation of the reaction conditions is usually required. Here we describe two approaches based on glutaraldehyde that readily give reliable results.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0003-2697
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
373
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
404-6
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Determination of protein oligomerization state: two approaches based on glutaraldehyde crosslinking.
pubmed:affiliation
Department of Biology, University of Crete, PO Box 2208, GR-71409 Heraklion, Crete, Greece.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't