Linked Life Data

18008148

Source:http://linkedlifedata.com/resource/pubmed/id/18008148

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2008-1-24
pubmed:abstractText
cAMP response element binding protein (CREB) belongs to ATF/CREB family of transcription factors, which are bonafide targets of cAMP-PKA signalling pathway in mammalian cells. CREB is known to regulate the genes involved in transcription, cell cycle, cell survival, neurotransmitter, growth factors and immune regulation. But there is no evidence of presence of ATF/CREB family members in Candida albicans. In the present study, CREB like transcription factor has been identified and purified in C. albicans. The putative CREB was observed to have different molecular mass (47 kDa) as compared to its mammalian counterpart (43 kDa). Both forms of CREB (CREB and phosphorylated CREB) were detected in C. albicans and phosphorylation of CREB was found to be a function of cAMP levels and protein kinase A activity within this organism. CREB protein was purified by sequence-specific CRE-DNA affinity chromatography. Purified CREB exhibited characteristic CRE binding activity as revealed by electrophoretic mobility shift assay and gave reactivity with CREB antibodies. CREB protein was phosphorylated by purified catalytic subunit of PKA under in vitro conditions. To the best of our knowledge, this study reports for the first time identification of CREB like protein as an important component of cAMP signalling pathway in C. albicans.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0300-8177
pubmed:author
pubmed:issnType
Print
pubmed:volume
308
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
237-45
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Identification and purification of CREB like protein in Candida albicans.
pubmed:affiliation
Department of Biochemistry, Postgraduate Institute of Medical Education & Research, Chandigarh 160 012, India.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't