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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2007-11-16
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pubmed:abstractText |
The sequence specific recognitions between DNAs and proteins play important roles in many biological functions. The use of double-stranded DNA arrays (ds-DNA arrays) for studying sequence specific recognition between DNAs and proteins is a promising method. Here we report the use of a ds-DNA probe with multi operation sites of restriction proteins in the middle sequence to investigate DNA-protein sequence-specific interactions including methylation. We arranged EcoR I site and Rsa I site on the same duplex DNA probe to fabricate ds-DNA arrays. We used the ds-DNA arrays to study DNA-restriction enzyme reactions before and after duplex DNA methylation under different probe concentration and reaction time conditions. Our results indicated that the ds-DNA arrays can be further biochemically modified and made accessible for interactions between DNAs and proteins in complex multi-step gene-regulation processes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1420-3049
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
417-26
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pubmed:dateRevised |
2009-10-27
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pubmed:meshHeading |
pubmed-meshheading:18007313-Base Sequence,
pubmed-meshheading:18007313-Binding Sites,
pubmed-meshheading:18007313-DNA,
pubmed-meshheading:18007313-DNA-Binding Proteins,
pubmed-meshheading:18007313-Deoxyribonuclease EcoRI,
pubmed-meshheading:18007313-Deoxyribonucleases, Type II Site-Specific,
pubmed-meshheading:18007313-Models, Biological,
pubmed-meshheading:18007313-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:18007313-Protein Binding,
pubmed-meshheading:18007313-Restriction Mapping,
pubmed-meshheading:18007313-Sequence Analysis, DNA,
pubmed-meshheading:18007313-Site-Specific DNA-Methyltransferase (Adenine-Specific),
pubmed-meshheading:18007313-Substrate Specificity
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pubmed:year |
2005
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pubmed:articleTitle |
Investigation of DNA-protein sequence-specific interactions with a ds-DNA array.
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pubmed:affiliation |
Chien-Shiung Wu Laboratory, Department of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Evaluation Studies
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