Linked Life Data

18007050

Source:http://linkedlifedata.com/resource/pubmed/id/18007050

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 11
pubmed:dateCreated
2007-11-16
pubmed:abstractText
The universal stress protein UspF (YnaF) is a small cytoplasmic bacterial protein. The expression of stress proteins is enhanced when cells are exposed to heat shock, nutrition starvation and certain other stress-inducing agents. YnaF promotes cell survival during prolonged exposure to stress and may activate a general mechanism for stress endurance. This manuscript reports preliminary crystallographic studies on YnaF from Salmonella typhimurium. The gene coding for YnaF was cloned and overexpressed and the protein was purified by Ni-NTA affinity chromatography. Purified YnaF was crystallized using vapour-diffusion and microbatch methods. The crystals belong to space group P2(1), with unit-cell parameters a = 37.51, b = 77.18, c = 56.34 A, beta = 101.8 degrees . A data set was collected to 2.5 A resolution with 94.6% completeness using an image-plate detector system mounted on a rotating-anode X-ray generator. Attempts to determine the structure are in progress.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18007050-11092928, http://linkedlifedata.com/resource/pubmed/commentcorrection/18007050-11567147, http://linkedlifedata.com/resource/pubmed/commentcorrection/18007050-11738040, http://linkedlifedata.com/resource/pubmed/commentcorrection/18007050-11849540, http://linkedlifedata.com/resource/pubmed/commentcorrection/18007050-1453957, http://linkedlifedata.com/resource/pubmed/commentcorrection/18007050-1532388, http://linkedlifedata.com/resource/pubmed/commentcorrection/18007050-15805601, http://linkedlifedata.com/resource/pubmed/commentcorrection/18007050-17081727, http://linkedlifedata.com/resource/pubmed/commentcorrection/18007050-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/18007050-5700707, http://linkedlifedata.com/resource/pubmed/commentcorrection/18007050-8152377, http://linkedlifedata.com/resource/pubmed/commentcorrection/18007050-8391533, http://linkedlifedata.com/resource/pubmed/commentcorrection/18007050-9860944
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1744-3091
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
63
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
957-60
pubmed:dateRevised
2010-9-15
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of universal stress protein F (YnaF) from Salmonella typhimurium.
pubmed:affiliation
Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560012, India.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't