Linked Life Data

18005101

Source:http://linkedlifedata.com/resource/pubmed/id/18005101

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2007-11-29
pubmed:abstractText
ICEBs1 is an integrative and conjugative element (conjugative transposon) integrated into trnS-leu2 in Bacillus subtilis. In response to DNA damage or high concentrations of potential mating partners, ICEBs1 can excise and transfer to various recipients, including other species. We found that excision of ICEBs1 occurs by site-specific recombination within 60 bp direct repeats that mark the junctions between ICEBs1 and chromosomal DNA. Excision required two ICEBs1 genes, int (integrase, ydcL), predicted to encode a tyrosine recombinase similar to that of phage lambda, and xis (excisionase, sacV). Ectopic expression of xis was sufficient to induce excision of ICEBs1, indicating that regulation of xis transcription by DNA damage and peptide signalling normally controls excision. Int, but not Xis, was needed for site-specific integration. We found that in the absence of the primary bacterial attachment site (attB) in trnS-leu2, ICEBs1 integrated in secondary attachment sites that are similar to a 17 bp sequence in attB. In the absence of int, ICEBs1 could recombine into the chromosome by RecA-dependent homologous recombination, provided ICEBs1 contained a region of sequence identity to a chromosomal locus.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0950-382X
pubmed:author
pubmed:issnType
Print
pubmed:volume
66
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1356-69
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Identification and characterization of int (integrase), xis (excisionase) and chromosomal attachment sites of the integrative and conjugative element ICEBs1 of Bacillus subtilis.
pubmed:affiliation
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural