Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
46
pubmed:dateCreated
2007-11-16
pubmed:abstractText
Understanding the fate of adult-generated neurons and the mechanisms that influence them requires consistent labeling and tracking of large numbers of stem cells. We generated a nestin-CreER(T2)/R26R-yellow fluorescent protein (YFP) mouse to inducibly label nestin-expressing stem cells and their progeny in the adult subventricular zone (SVZ) and subgranular zone (SGZ). Several findings show that the estrogen ligand tamoxifen (TAM) specifically induced recombination in stem cells and their progeny in nestin-CreER(T2)/R26R-YFP mice: 97% of SGZ stem-like cells (GFAP/Sox2 with radial glial morphology) expressed YFP; YFP+ neurospheres could be generated in vitro after recombination in vivo, and maturing YFP+ progeny were increasingly evident in the olfactory bulb (OB) and dentate gyrus (DG) granule cell layer. Revealing an unexpected regional dissimilarity in adult neurogenesis, YFP+ cells accumulated up to 100 d after TAM in the OB, but in the SGZ, YFP+ cells reached a plateau 30 d after TAM. In addition, most SVZ and SGZ YFP+ cells became neurons, underscoring a link between nestin and neuronal fate. Finally, quantification of YFP+ cells in nestin-CreER(T2)/R26R-YFP mice allowed us to estimate, for example, that stem cells and their progeny contribute to no more than 1% of the adult DG granule cell layer. In addition to revealing the dynamic contribution of nestin-expressing stem cells to adult neurogenesis, this work highlights the utility of the nestin-CreER(T2)/R26R-YFP mouse for inducible gene ablation in stem cells and their progeny in vivo in the two major regions of adult neurogenesis.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1529-2401
pubmed:author
pubmed:issnType
Electronic
pubmed:day
14
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
12623-9
pubmed:dateRevised
2011-4-28
pubmed:meshHeading
pubmed-meshheading:18003841-Animals, pubmed-meshheading:18003841-Brain, pubmed-meshheading:18003841-Cell Differentiation, pubmed-meshheading:18003841-Cell Lineage, pubmed-meshheading:18003841-Cell Proliferation, pubmed-meshheading:18003841-Dentate Gyrus, pubmed-meshheading:18003841-Gene Targeting, pubmed-meshheading:18003841-Intermediate Filament Proteins, pubmed-meshheading:18003841-Luminescent Proteins, pubmed-meshheading:18003841-Mice, pubmed-meshheading:18003841-Mice, Transgenic, pubmed-meshheading:18003841-Models, Animal, pubmed-meshheading:18003841-Nerve Regeneration, pubmed-meshheading:18003841-Nerve Tissue Proteins, pubmed-meshheading:18003841-Neuroglia, pubmed-meshheading:18003841-Neurons, pubmed-meshheading:18003841-Olfactory Bulb, pubmed-meshheading:18003841-Recombinant Fusion Proteins, pubmed-meshheading:18003841-Recombination, Genetic, pubmed-meshheading:18003841-Selective Estrogen Receptor Modulators, pubmed-meshheading:18003841-Stem Cells, pubmed-meshheading:18003841-Tamoxifen
pubmed:year
2007
pubmed:articleTitle
Dynamic contribution of nestin-expressing stem cells to adult neurogenesis.
pubmed:affiliation
Department of Psychiatry, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9070, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural