Linked Life Data

18001269

Source:http://linkedlifedata.com/resource/pubmed/id/18001269

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2008-4-1
pubmed:abstractText
Previous studies have provided conflicting conclusions concerning the efficacy of improving protein balance in patients by standard intravenous nutrition [TPN (total parenteral nutrition)], which is either explained by suboptimal nutritional regimens or insensitive clinical methods. The aim of the present study was therefore to evaluate the effects on the initiation of translation of skeletal muscle proteins by standard overnight TPN. A total of 12 patients who underwent standard surgery were included. TPN was provided as an all-in-one treatment by constant infusion [0.16 gN.kg(-1) of body weight.day(-1) (30 kcal.kg(-1) of body weight.day(-1))]. Saline-infused patients served as controls. Rectus abdominis muscle biopsies were taken at the time of the operation. The phosphorylation state of the proteins for initiation of translation was quantified. Plasma glucose, and serum insulin, glycerol, triacylglycerols (triglycerides) and NEFAs (non-esterified fatty acids; 'free fatty acids') were not significantly altered during TPN infusion, whereas total plasma amino acids increased, as shown by increases in methionine, phenylalanine, threonine, alanine, arginine, aspartic acid, glycine and histidine (P<0.05). Overnight TPN increased the formation of active eIF4G-eIF4E (where eIF is eukaryotic-initiation factor) complexes (P<0.05), whereas the inhibitory complex 4E-BP1 (eIF4E-binding protein)-eIF4E was moderately decreased (P<0.06). TPN increased the amount of the most phosphorylated form of 4E-BP1 (P<0.05), and increased the amount (P<0.04) and phosphorylation (P<0.01) of p70(S6K) (70 kDa ribosomal protein S6 kinase). In conclusion, an overnight pre-operative constant infusion of standard TPN altered initiation factor complexes, indicating activation of the initiation of protein translation in rectus abdominis muscle in the presence of increased plasma amino acid levels, but without a concomitant increase in energy substrates and insulin. In contrast with our results from previous studies, the methodology used in the present study appears to be more sensitive in reflecting directional changes in human muscle protein synthesis compared with traditional methods, particularly based on measurements of amino acid flux.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1470-8736
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
114
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
603-10
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:18001269-Adaptor Proteins, Signal Transducing, pubmed-meshheading:18001269-Aged, pubmed-meshheading:18001269-Amino Acids, pubmed-meshheading:18001269-Biopsy, pubmed-meshheading:18001269-Case-Control Studies, pubmed-meshheading:18001269-Eukaryotic Initiation Factor-4E, pubmed-meshheading:18001269-Eukaryotic Initiation Factor-4G, pubmed-meshheading:18001269-Female, pubmed-meshheading:18001269-Humans, pubmed-meshheading:18001269-Insulin, pubmed-meshheading:18001269-Male, pubmed-meshheading:18001269-Middle Aged, pubmed-meshheading:18001269-Neoplasms, pubmed-meshheading:18001269-Parenteral Nutrition, pubmed-meshheading:18001269-Peptide Initiation Factors, pubmed-meshheading:18001269-Phosphoproteins, pubmed-meshheading:18001269-Phosphorylation, pubmed-meshheading:18001269-Protein Biosynthesis, pubmed-meshheading:18001269-Rectus Abdominis, pubmed-meshheading:18001269-Ribosomal Protein S6 Kinases, 70-kDa
pubmed:year
2008
pubmed:articleTitle
Initiation factors for translation of proteins in the rectus abdominis muscle from patients on overnight standard parenteral nutrition before surgery.
pubmed:affiliation
Department of Surgery, Surgical Metabolic Research Laboratory at Lundberg Laboratory for Cancer Research, Sahlgrenska University Hospital, SE413 45 Göteborg, Sweden.
pubmed:publicationType
Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't