Source:http://linkedlifedata.com/resource/pubmed/id/17998933
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
19
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pubmed:dateCreated |
2008-4-24
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pubmed:abstractText |
Histone methylation is involved in the regulation of gene expression and DNA replication through alteration of chromatin structure. We earlier showed that SMYD3, a histone H3-lysine 4-specific methyltransferase, is frequently upregulated in human colorectal, liver and breast cancer compared to their matched non-cancerous cells, and that its activity is associated with the growth of these tumors. In the present study, we found that human cancer cells express both the full-length and a cleaved form of SMYD3 protein. Amino acid sequence analysis uncovered that the cleaved form lacks the 34 amino acids in the N-terminal region of the full-length protein. Interestingly, the cleaved protein and mutant protein containing substitutions at glycines 15 and 17, two highly conserved amino acids in the N-terminal region, revealed a higher histone methyltransferase (HMTase) activity compared to the full-length protein. Furthermore, the N-terminal region is responsible for the association with heat shock protein 90alpha (HSP90alpha). These data indicate that the N-terminal region plays an important role for the regulation of its methyltransferase activity and suggest that a structural change of the protein through the cleavage of the region or interaction with HSP90alpha may be involved in the modulation. These findings may help for a better understanding of the mechanisms that modulate the HMTase activity of SMYD3, and contribute to the development of novel anticancer drugs targeting SMYD3 methyltransferase activity.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1476-5594
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:day |
24
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pubmed:volume |
27
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2686-92
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pubmed:meshHeading |
pubmed-meshheading:17998933-Amino Acid Sequence,
pubmed-meshheading:17998933-Cell Line,
pubmed-meshheading:17998933-Cell Line, Tumor,
pubmed-meshheading:17998933-HCT116 Cells,
pubmed-meshheading:17998933-Histone-Lysine N-Methyltransferase,
pubmed-meshheading:17998933-Humans,
pubmed-meshheading:17998933-Molecular Sequence Data,
pubmed-meshheading:17998933-Neoplasm Proteins,
pubmed-meshheading:17998933-Peptides,
pubmed-meshheading:17998933-Protein Structure, Tertiary
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pubmed:year |
2008
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pubmed:articleTitle |
Enhanced methyltransferase activity of SMYD3 by the cleavage of its N-terminal region in human cancer cells.
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pubmed:affiliation |
Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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