Source:http://linkedlifedata.com/resource/pubmed/id/17994907
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
2007-11-12
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pubmed:abstractText |
We developed a novel scheme for two-photon fluorescence bioimaging. We generated supercontinuum (SC) light at wavelengths of 600 to 1200 nm with 774-nm light pulses from a compact turn-key semiconductor laser picosecond light pulse source that we developed. The supercontinuum light was sliced at around 1030- and 920-nm wavelengths and was amplified to kW-peak-power level using laboratory-made low-nonlinear-effects optical fiber amplifiers. We successfully demonstrated two-photon fluorescence bioimaging of mouse brain neurons containing green fluorescent protein (GFP).
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:issn |
1083-3668
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pubmed:author |
pubmed-author:GuoHengchangH,
pubmed-author:ItoHiromasaH,
pubmed-author:KashiwagiKaoriK,
pubmed-author:SaitoNaoakiN,
pubmed-author:SatoKi-ichiK,
pubmed-author:ShikataJun-ichiJ,
pubmed-author:TakashimaKeijiroK,
pubmed-author:TaniguchiHirokazuH,
pubmed-author:TsubokawaHiroshiH,
pubmed-author:YokoyamaHiroyukiH
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pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
054019
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pubmed:meshHeading |
pubmed-meshheading:17994907-Animals,
pubmed-meshheading:17994907-Brain,
pubmed-meshheading:17994907-Equipment Design,
pubmed-meshheading:17994907-Equipment Failure Analysis,
pubmed-meshheading:17994907-Image Enhancement,
pubmed-meshheading:17994907-Lasers,
pubmed-meshheading:17994907-Mice,
pubmed-meshheading:17994907-Microscopy, Fluorescence, Multiphoton,
pubmed-meshheading:17994907-Neurons,
pubmed-meshheading:17994907-Semiconductors
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pubmed:articleTitle |
Two-photon bioimaging utilizing supercontinuum light generated by a high-peak-power picosecond semiconductor laser source.
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pubmed:affiliation |
Tohoku University, New Industry Creation Hatchery Center (NICHe), 6-6-10 Aramaki-Aoba, Aoba-ku, Sendai 980-8579, Japan. yoko@niche.tohoku.ac.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Evaluation Studies
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