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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2008-3-14
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pubmed:abstractText |
Conditions that influence the selective development or recruitment of connective tissue-type and mucosal-type mast cells (MCs) are not well understood. Here, we report that cynomolgus monkey embryonic stem (ES) cells cocultured with the murine aorta-gonad-mesonephros-derived stromal cell line AGM-S1 differentiated into cobblestone (CS)-like cells by day 10-15. When replated onto fresh AGM-S1 with the addition of stem cell factor, interleukin-6, and Flt3 ligand, these CS-like cells displayed robust growth and generated almost 100% tryptase/chymase double-positive MCs within 3 weeks. At all time points, the percentage of tryptase-positive cells did not exceed that of chymase-positive cells. These ES-derived MCs were CD45+/Kit+/CD31+/CD203c+/HLA-DR- and coexpressed a high-affinity IgE receptor on their surface, which was upregulated after IgE exposure. Electron microscopy showed that they contained many electron dense granules. Moreover, ES-derived MCs responded to stimulation by via IgE and substance P by releasing histamine. These results indicate that ES-derived MCs have the phenotype of functionally mature connective tissue-type MCs. The rapid maturation of ES-derived MCs suggests a unique embryonic pathway in primates for early development of connective tissue-type MCs, which may be independent from the developmental pathway of mucosal-type MCs.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1549-4918
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pubmed:author |
pubmed-author:DengL JLJ,
pubmed-author:FujinoHisanoriH,
pubmed-author:FujisawaAkihiroA,
pubmed-author:HeikeToshioT,
pubmed-author:KambeNaotomoN,
pubmed-author:MaLinaL,
pubmed-author:MiyachiYoshikiY,
pubmed-author:NakahataTatsutoshiT,
pubmed-author:NakatsujiNorioN,
pubmed-author:ShinodaGenG,
pubmed-author:SuemoriHirofumiH,
pubmed-author:ToriiRyuzoR,
pubmed-author:TsujiKohichiroK,
pubmed-author:UmedaKatsutsuguK,
pubmed-author:WangDanD
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pubmed:issnType |
Electronic
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pubmed:volume |
26
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
706-14
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pubmed:meshHeading |
pubmed-meshheading:17991916-Animals,
pubmed-meshheading:17991916-Cell Differentiation,
pubmed-meshheading:17991916-Cell Line,
pubmed-meshheading:17991916-Cell Proliferation,
pubmed-meshheading:17991916-Chymases,
pubmed-meshheading:17991916-Coculture Techniques,
pubmed-meshheading:17991916-Cytokines,
pubmed-meshheading:17991916-Embryonic Stem Cells,
pubmed-meshheading:17991916-Flow Cytometry,
pubmed-meshheading:17991916-Hematopoietic Stem Cells,
pubmed-meshheading:17991916-Histamine,
pubmed-meshheading:17991916-Humans,
pubmed-meshheading:17991916-Immunoglobulin E,
pubmed-meshheading:17991916-Mast Cells,
pubmed-meshheading:17991916-Mice,
pubmed-meshheading:17991916-Multipotent Stem Cells,
pubmed-meshheading:17991916-Phenotype,
pubmed-meshheading:17991916-Primates,
pubmed-meshheading:17991916-Substance P,
pubmed-meshheading:17991916-Tryptases
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pubmed:year |
2008
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pubmed:articleTitle |
Direct development of functionally mature tryptase/chymase double-positive connective tissue-type mast cells from primate embryonic stem cells.
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pubmed:affiliation |
Department of Pediatrics, Kyoto University Graduate School of Medicine, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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