Linked Life Data

17990880

Source:http://linkedlifedata.com/resource/pubmed/id/17990880

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
49
pubmed:dateCreated
2007-12-6
pubmed:abstractText
We report the results from a new solid-state NMR experiment, DANTE-REDOR, which can determine global secondary structure in uniformly (13C,15N)-enriched systems by simultaneously measuring distance and orientation constraints. Following a heteronuclear spin-pair selection using a DANTE pulse train, the magnitude and orientation of the internuclear dipole vector, within the chemical shift anisotropy (CSA) frame of the observed nucleus, are determined by tracking the dephasing of individual spinning sidebands under magic angle spinning. The efficacy of the experiment is demonstrated by measuring the imidazole side-chain orientation in U-[13C6,15N3]-L-histidine x HCl x H2O.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1520-5126
pubmed:author
pubmed:issnType
Electronic
pubmed:day
12
pubmed:volume
129
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
15233-9
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Determination of global structure from distance and orientation constraints in biological solids using solid-state NMR spectroscopy.
pubmed:affiliation
Department of Chemistry and Biochemistry, Oberlin College, Oberlin, Ohio 44074, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.