Linked Life Data

17990558

Source:http://linkedlifedata.com/resource/pubmed/id/17990558

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2007-11-9
pubmed:abstractText
One variant of microcystins was isolated and purified with cyanobacteria natural bloom as the starting material, which was collected in Dianchi Lake, China. The separation protocol involved extraction of cyanobacterial cells by 75% aqueous methanol, isolation by reversed-phase flash chromatography, and purification by reversed-phase semipreparative HPLC. The structure and purity of purified microcystin was identified with electrospray ionization mass spectrometry, UV spectrophotometer, and analytical HPLC. The purified microcystin was assigned as [Dha7]MCRR (purity > 95%), which was a demethylated variant of MCRR. The structure of purified microcystin was identified as cyclo-(Ala-Arg-MeAsp-Arg-Adda-Glu-Dha) with molecular weight of 1023. There was a maximum absorbance at 239 nm in its UV spectrum (200-300 nm). This variant of microcystins occurred frequently, and sometimes could become the main variant in waterbloom from Dianchi Lake.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0250-3301
pubmed:author
pubmed:issnType
Print
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2063-7
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
[Preparative purification and characterization of [Dha7] microcystin RR].
pubmed:affiliation
College of Resources and Environmental Engineering, Wuhan University of Technology, Wuhan 430070, China. xiaoguo_chen@tom.com
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't