Linked Life Data

17989959

Source:http://linkedlifedata.com/resource/pubmed/id/17989959

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2007-12-24
pubmed:abstractText
We used Fourier transform infrared spectromicroscopy in the attenuated total reflection configuration to study biochemical events associated with the response to light of an intact retina. We show that the technique is suitable for the detection in real time of molecular processes occurring in rod outer segments induced by light absorption. Two-dimensional correlation analysis was applied to the identification and interpretation of specific spectral changes associated to the evolution of the system. The technique allows us to observe an extensive protein translocation, which we interpret as arising from the release of transducin from the disk membrane and its redistribution from the outer segment towards the inner segment of rod cells. These results are in full agreement with our current understanding of retinal physiology and validate the technique as a useful tool for the study of complex molecular processes in intact tissue. [figure: see text] Spectral changes in the mid infrared region following exposure of an intact retina to light.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1618-2650
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
390
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
317-22
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Detection of molecular processes in the intact retina by ATR-FTIR spectromicroscopy.
pubmed:affiliation
International School for Advanced Studies (SISSA), Via Beirut 2, 34024, Trieste, Italy.
pubmed:publicationType
Journal Article