Linked Life Data

17989187

Source:http://linkedlifedata.com/resource/pubmed/id/17989187

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2008-1-8
pubmed:abstractText
Quantification of Epstein-Barr virus (EBV) in peripheral blood is important for the diagnosis and management of serious EBV diseases, including posttransplant lymphoproliferative disorder. A variety of PCR-based methods are currently in use; however, there is little information on their comparability. This study assessed the relative performance of different quantitative assays. A multicenter comparative study was performed at eight sites using three panels consisting of serial dilutions of quantified EBV DNA and extracts from a total of 19 whole-blood specimens. Samples were distributed and tested blindly. Instrumentation, probe chemistries, amplification targets, and other test-related aspects varied considerably between laboratories. Each laboratory's calibration curve indicated strong evidence of a consistent log-linear relationship between viral load and cycle threshold, suggesting that intralaboratory tracking of a given patient would yield similar relative quantitative trends among the participating test sites. There was strong concordance among laboratories with respect to qualitative test results; however, marked quantitative discordance was seen. For most samples, the across-laboratory interquartile range of the reported viral load (in copies/microl) was roughly 0.6 log-units, and for one sample the overall range was approximately 4.2 log-units. While intralaboratory tracking of patients may yield similar results, these data indicate a need for caution when attempting to compare clinical results obtained at different institutions and suggest the potential value to be gained by more standardized testing methodology.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17989187-10068600, http://linkedlifedata.com/resource/pubmed/commentcorrection/17989187-10341329, http://linkedlifedata.com/resource/pubmed/commentcorrection/17989187-11163581, http://linkedlifedata.com/resource/pubmed/commentcorrection/17989187-11226561, http://linkedlifedata.com/resource/pubmed/commentcorrection/17989187-11339072, http://linkedlifedata.com/resource/pubmed/commentcorrection/17989187-11856464, http://linkedlifedata.com/resource/pubmed/commentcorrection/17989187-11884626, http://linkedlifedata.com/resource/pubmed/commentcorrection/17989187-12153173, http://linkedlifedata.com/resource/pubmed/commentcorrection/17989187-14605174, http://linkedlifedata.com/resource/pubmed/commentcorrection/17989187-15494263, http://linkedlifedata.com/resource/pubmed/commentcorrection/17989187-15938741, http://linkedlifedata.com/resource/pubmed/commentcorrection/17989187-16095501, http://linkedlifedata.com/resource/pubmed/commentcorrection/17989187-17016878, http://linkedlifedata.com/resource/pubmed/commentcorrection/17989187-17318076, http://linkedlifedata.com/resource/pubmed/commentcorrection/17989187-17494720, http://linkedlifedata.com/resource/pubmed/commentcorrection/17989187-9811515
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1098-660X
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
157-63
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Multicenter comparison of different real-time PCR assays for quantitative detection of Epstein-Barr virus.
pubmed:affiliation
Department of Pathology, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't, Multicenter Study