Linked Life Data

17987463

Source:http://linkedlifedata.com/resource/pubmed/id/17987463

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
14
pubmed:dateCreated
2007-11-7
pubmed:abstractText
Ambient fine particulate matter has been associated with cardiovascular and other diseases in epidemiological studies, and diesel exhaust (DE) is a major source of urban fine particulate matter. Air pollution's cardiovascular effects have been attributed to oxidative stress and systemic inflammation, with resulting perturbation of vascular homeostasis. Peripheral leukocytes are involved in both inflammation and control of vascular homeostasis. We conducted a pilot study using microarray techniques to analyze whether global gene expression profiles in peripheral blood mononuclear cells (PBMCs) can elucidate effects of DE inhalation, for further investigation of mechanisms underlying vascular effects. In a double-blind, crossover, controlled exposure study, healthy adult volunteers were exposed in randomized order to filtered air (FA) and diluted DE in 2-h sessions. We isolated RNA (Trizol/Qiagen method) from PBMCs before and two times after each exposure. RNA samples were arrayed using the Affymetrix U133 Plus 2.0 arrays. Microarray analyses were conducted on five subjects with available RNA samples from exposures to FA and to the highest DE inhalation (200 microg/m(3) of fine particulate matter). Following data normalization and statistical analysis, a total of 1290 out of 54,675 probe sets evidenced differential expression (more than 1.5-fold up- or downregulated with p < .05) between FA and DE exposure. These genes demonstrated a clear distinction between the FA and DE groups and an indication of a time-dependent effect on biological processes such as inflammation and oxidative stress. This study addresses the value of using PBMC gene expression to assess pathways relevant to cardiovascular effect in healthy individuals.
pubmed:grant
http://linkedlifedata.com/resource/pubmed/grant/ES013195, http://linkedlifedata.com/resource/pubmed/grant/K23ES011139, http://linkedlifedata.com/resource/pubmed/grant/K24 ES013195-01A1, http://linkedlifedata.com/resource/pubmed/grant/K24 ES013195-02, http://linkedlifedata.com/resource/pubmed/grant/K24 ES013195-03, http://linkedlifedata.com/resource/pubmed/grant/K24ES013195, http://linkedlifedata.com/resource/pubmed/grant/M01 RR000037-440886, http://linkedlifedata.com/resource/pubmed/grant/M01 RR000037-450886, http://linkedlifedata.com/resource/pubmed/grant/M01 RR000037-450972, http://linkedlifedata.com/resource/pubmed/grant/M01 RR000037-460972, http://linkedlifedata.com/resource/pubmed/grant/M01 RR000037-475376, http://linkedlifedata.com/resource/pubmed/grant/M01RR-00037, http://linkedlifedata.com/resource/pubmed/grant/P30 ES007033-119016, http://linkedlifedata.com/resource/pubmed/grant/P30 ES007033-129016, http://linkedlifedata.com/resource/pubmed/grant/P30 ES007033-139016, http://linkedlifedata.com/resource/pubmed/grant/P30 ES007033-149016, http://linkedlifedata.com/resource/pubmed/grant/P30ES07033
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1091-7691
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1107-19
pubmed:dateRevised
2011-4-25
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Diesel exhaust inhalation and assessment of peripheral blood mononuclear cell gene transcription effects: an exploratory study of healthy human volunteers.
pubmed:affiliation
Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, Washington, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S., Randomized Controlled Trial, Research Support, N.I.H., Extramural