Linked Life Data

17980352

Source:http://linkedlifedata.com/resource/pubmed/id/17980352

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2007-12-6
pubmed:abstractText
Lipid rafts are small cholesterol- and glycosphingolipid-enriched membrane subdomains. Here we compared the mu opioid receptor (MOR)-lipid rafts relationship in the rat brain, where neurons have non-caveolae rafts, and in CHO cells stably transfected with HA-rat MOR (CHO-HA-rMOR), which are enriched in caveolae. Membranes of rat caudate putamen (CPu) and thalamus or CHO-HA-rMOR cells were homogenized, sonicated in a detergent-free 0.5 M Na(2)CO(3) buffer and fractionated through sucrose density gradients. Western blot and [(3)H]diprenorphine binding showed that approximately 70% of MOR in CHO-HA-rMOR was present in low-density (5-20% sucrose) fractions enriched in cholesterol and/or ganglioside M1 (GM1) (lipid rafts) in plasma membranes, whereas about 70% and 45% of MOR in CPu and thalamus, respectively, were associated with lipid rafts. Incubation with a saturating concentration of etorphine or morphine at 37 degrees C for 30 min failed to change the MOR location in rafts in CHO-HA-rMOR, indicating that the internalized MOR does not move out of rafts, in contrast to the delta opioid receptor. In vivo, rafts association of MOR in CPu and thalamus was not affected significantly in rats implanted with two 75-mg morphine pellets for 72 h. In addition, cholesterol reduction by methyl-beta-cyclodextrin (MCD) disrupted rafts and shifted MOR to higher density fractions in both CHO-HA-rMOR and CPu membranes. However, MCD treatment had opposite impacts on MOR signaling in the two tissues: it attenuated MOR-mediated [(35)S]GTPgammaS binding in CPu but enhanced it in CHO-HA-rMOR.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:day
12
pubmed:volume
1184
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
46-56
pubmed:meshHeading
pubmed-meshheading:17980352-Animals, pubmed-meshheading:17980352-Binding, Competitive, pubmed-meshheading:17980352-Brain, pubmed-meshheading:17980352-CHO Cells, pubmed-meshheading:17980352-Caveolin 1, pubmed-meshheading:17980352-Cholesterol, pubmed-meshheading:17980352-Cricetinae, pubmed-meshheading:17980352-Cricetulus, pubmed-meshheading:17980352-Diprenorphine, pubmed-meshheading:17980352-Dose-Response Relationship, Drug, pubmed-meshheading:17980352-Etorphine, pubmed-meshheading:17980352-Male, pubmed-meshheading:17980352-Membrane Microdomains, pubmed-meshheading:17980352-Morphine, pubmed-meshheading:17980352-Naloxone, pubmed-meshheading:17980352-Narcotic Antagonists, pubmed-meshheading:17980352-Protein Binding, pubmed-meshheading:17980352-Rats, pubmed-meshheading:17980352-Rats, Sprague-Dawley, pubmed-meshheading:17980352-Receptors, Opioid, mu, pubmed-meshheading:17980352-Signal Transduction, pubmed-meshheading:17980352-Transfection
pubmed:year
2007
pubmed:articleTitle
Agonist treatment did not affect association of mu opioid receptors with lipid rafts and cholesterol reduction had opposite effects on the receptor-mediated signaling in rat brain and CHO cells.
pubmed:affiliation
Department of Pharmacology, Temple University School of Medicine, Philadelphia, PA 19140, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural