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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2007-11-21
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pubmed:abstractText |
GPR40 is G protein-coupled receptor whose endogenous ligands have recently been identified as free fatty acids (FFAs), and it has been implicated to play an important role in FFA-mediated enhancement of glucose-stimulated insulin release. We have developed a monoclonal antibody against the extracellular domain of GPR40. Specificity of the antibody was demonstrated by immunoprecipitation and cell surface staining using GPR40-transfected cells. GPR40 immunoreactivity was highly abundant in mouse pancreatic beta-cells and splenocytes, THP-1 cells, and human peripheral blood mononuclear cells. The anti-GPR40 monoclonal antibody should prove valuable for further studying the function of this nutrient sensing receptor.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1090-2104
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
4
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pubmed:volume |
365
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
22-8
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pubmed:meshHeading |
pubmed-meshheading:17980148-Amino Acid Sequence,
pubmed-meshheading:17980148-Animals,
pubmed-meshheading:17980148-Antibodies, Monoclonal,
pubmed-meshheading:17980148-Antibody Specificity,
pubmed-meshheading:17980148-Cells, Cultured,
pubmed-meshheading:17980148-Humans,
pubmed-meshheading:17980148-Immunohistochemistry,
pubmed-meshheading:17980148-Leukocytes, Mononuclear,
pubmed-meshheading:17980148-Mice,
pubmed-meshheading:17980148-Molecular Sequence Data,
pubmed-meshheading:17980148-Peptide Fragments,
pubmed-meshheading:17980148-Receptors, G-Protein-Coupled,
pubmed-meshheading:17980148-Transfection
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pubmed:year |
2008
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pubmed:articleTitle |
Production and characterization of a monoclonal antibody against GPR40 (FFAR1; free fatty acid receptor 1).
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pubmed:affiliation |
Department of Genomic Drug Discovery Science, Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29 Yoshida-shimo-adachi-cho, Sakyo-ku, Kyoto 606-8501, Japan. akira_h@pharm.kyoto-u.ac.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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